牛磺酸減緩狼瘡小鼠肝纖維化及環氧合蛋白質表現

Purpose: There is growing evidence of a possible association between high-cholesterol diet and hepatic abnormality in systemic lupus erythematosus (SLE). To investigate the effects of taurine on the hepatic abnormality in SLE, NZB/W F1 mice were randomly divided into three groups: a control group, cholesterol group, and a cholesterol plus taurine group.Methods: Hematoxylin-Eosin staining and Immunoblots were used to perform the analysis.Results: Mice treated with cholesterol plus taurine had improvements in both hepatic cleft and irregular architecture in the liver, compared to the other two groups. Additionally, α-smooth muscle actin (α-SMA), the hepatic associated factor, was significantly more decreased in mice treated with cholesterol and taurine than in those of the other two groups.Moreover, mice treated with cholesterol and taurine had significant greater decrease in Cyclooxygenase-2 (Cox-2) protein in the liver than the other groups.Conclusions: Our findings suggested that taurine is a beneficial dietary supplement for alleviating hepatic fibrosis and inflammation in SLE.
目的：目前已有許多證據指出，高膽固醇食物與紅斑性狼瘡病人中，肝臟異常之關聯性。為暸解牛磺酸對紅斑性狼瘡肝臟異常的影響，我們將NZB/W F1小鼠隨機分為三組，分別餵食對照食物、膽固醇食物、牛磺酸加上膽固醇食物。方法：我們採用蘇木紫－伊紅染色法以及西方墨點法來進行相關的分析。結果：實驗結果發現餵食膽固醇加上牛磺酸食物的狼瘡小鼠比起餵食膽固醇食物的小鼠，其肝臟細胞呈現較規則排列，且肝臟中纖維化相關蛋白質α-SMA的表現顯著降低，此外，重要的發炎相關蛋白質及環氧合?也呈現顯著下降。結論：這些實驗結果證明牛磺酸能減緩狼瘡小鼠肝臟纖維化蛋白質及環氧合?的表現。