毛細管電泳在生物醫學上的應用

中文摘要 (一) 尼古丁化合物經常使用臨床醫學設備來監測其在血清中的濃 度 , 利用微胞電動利毛細管電泳法 ( MECC ) 在磷酸 — 硼酸緩衝液系統下進行測定 。使用SDS 的微胞系統配合適當的有機修飾劑 ( Acetonitrile ) , 成功地解析出此種尼古丁化合物 。這類冲堤模式可使尼古丁在 30 KV的電壓下於四分鐘內被冲堤出來 。改變施加電壓 、 溫度 、 有機修飾劑及界面活性劑對於移動行為的影響亦被研究 。 (二) 大蒜精是一般常見的食品 , 就目前所研究的結果顯示大蒜精具有多種的特殊功能 , 例如能針對許多不同的腫瘤產生有效的抑制 , 又或能降低血脂及增加抗凝血功能等 。利用毛細管電層析法於各種不同的有機修飾劑 、 界面活性濟及緩衝溶液系統中進行試驗分析 。 有效分離大蒜精中三種主要活性成分 DAS 、 DADS 、 DATS 的最適當 CE 分析條件尚未完成 , 必須作更進一步的研究 。

Abstract (1) The nicotine compound typically employed in clinical / medical settings for monitoring its concentration in the serum is determined by Micellar Electrokinetic Capillary Chromatography ( MECC ) in phosphate - borate buffer . Successful resolution of such compound was achieved with use of a micelle system composed of sodium dodecyl sulfate in conjunction with an appropriate organic modifier ( acetonitrile ) . The elution pattern of nicotine can be obtained within four minutes with a voltage of 30 KV . The effect of varying the applied voltage , temperature and the organic modifiers with surfactants on the migration behavior is also examined . (2) Garlic is common food . Allicin is main component of garlic . It can increase the function of antiplatelet activity and prevent tumors from forming , and so on . Varying organic solvents , surfactants , and buffer systems in the experimental analysis were tested . The most appropriate analytical condition of the three major elements , DAS 、 DADS and DATS of allicin is still understudied .