Isopentylamine導致黑色素腫瘤細胞死亡機制之探討

異戊胺（Isopentylamine）是一種脂肪族的單胺，由奇異變形桿菌（Proteus mirabilis）產生，也存在於西非尼日利亞的可樂果品種中，生活當中，最常見的Isopentylamine是存在於乳酪和葡萄酒中。研究指出，Isopentylamine是由L-leucine經由Leucine decarboxylase所產生的。而Leucine decarboxylase則是由Murine Gm853(LDC)基因產生，或是ODC產生相似的酵素。目前關於Isopentylamine的生物效應現有數據較少。黑色素瘤，也稱為惡性黑色素瘤，是一種黑色素細胞發展而來的癌症。研究指出，皮膚黑色素瘤有80％以上是由RAS/RAF/MEK/ERK途徑所激活的。由於Isopentylamine的存在與調節細胞週期有關，為了釐清Isopentylamine是否會參與可能的調節機制，我們用不同濃度的Isopentylamine處理B16-F1黑色素瘤細胞。實驗數據顯示，B16-F1細胞會隨著處理Isopentylamine的濃度上升，使B16-F1細胞存活率減少，並且抑制調節細胞生存的MEK/MSK訊息路徑，導致細胞生長相關調節蛋白被抑制，而讓細胞數減少。另外RSK會引導LKB1上升和AMPK/FASN的訊息傳導路徑，讓細胞走向autophagy的路徑。這是值得探討的細節，或許Isopentylamine可以成為一個治療黑色素腫瘤的有效方式。

Isopentylamine, an aliphatic monoamine, is produced byProteus mirabilis and present in kola nut varieties of Nigerian origin. The most common Isopentylamine in our lives is found in cheese and wine. Leucine decarboxylase is produced by the Murine Gm853 (LDC)gene, or ODC produces similar enzymes.At present, there are less available data on the possible biological effects of Isopentylamin. Melanoma, also known asmalignant melanoma, is atype of cancerthat develops from melanocytes.The RAS/RAF/MEK/ERK pathway has been reported to be activated in over 80 percent of all cutaneous melanomas.Since the presence of Isopentylamineis related to cell proliferation, expect to clarify the possible regulatory mechanisms,we treated B16-F1 cells with different concentrations of Isopentylamine. For the data show, B16-F1 cells decrease survival as the concentration of Isopentylamine is increased, and MEK/MSK pathway that regulates cell survival is reppressed, resulting in inhibition of cell growth and decrease in cell number. In addition, decline of RSK lead to riseof LKB1 and then regulate AMPK/FASN signal pathway that inducing cell autophagy.This is worth to explore the details, Isopentylamine may can be an effective way to treat melanoma.