應用NCBI網路系統搜尋新穎的酵素-WWOX

應用NCBI網路系統搜尋新穎的酵素-WWOX 摘要 紀璵繡 中山醫學大學營養科學研究所 NCBI (National Center of Biotechnology Information)網路搜尋系統，是獲取生物資訊最常用的資料庫，本研究即利用此系統工具搜尋到一個人類的新穎酵素WWOX(ww domain containing oxidoreductase, NM_016373 and NP_057457) 。並且為未來的動用實驗考量搜尋到在斑馬魚中與之對應的酵素(BC 044560 and AAH44560)。人類的WWOX基因坐落於染色體 16q23.3-q24.1，由9個exon組成，編碼區域含有1245個核酸，414個胺基酸，具有二個 WW domain 及一個short chain dehydrogenase/reductase (SDR) domain。而斑馬魚的WWOX基因編碼區域則含有1239個核酸，412個胺基酸。 WWOX廣泛的分佈於人類的腦部、肺部、肝臟、胰臟、腎臟、以及前列腺。過去的研究顯示，該基因可能是屬於抑癌基因，例如: 改變WWOX的基因與非小細胞的肺癌(Non-small cell lung cancer)之發生有關。另外，最近也發現WWOX於發育中之鼠科動物的神經系統中含量豐富。若依WWOX的蛋白質功能區推測，WW domain與細胞內蛋白質間的交互作用有關，而SRD與性固醇(sex steroid)代謝有關。雖然與WWOX相關的研究很多，但就其詳細功能及生理機轉卻是未知的，仍有待進一步之探討。

Searching Novel Enzyme by Internet Tool of the NCBI Databases-WWOX Yu-Hsiu Chi Abstract In this study, the search tool BLAST (Basic Local Alignment Search Tool), based on NCBI (National Center for Biotechnology Information) was used against nucleotide and protein databases. The analysis found a ww domain containing oxidoreductase (WWOX, NCBI accession number NM_016373 and NP_057457) and zebrafish (NCBI accession number BC 044560 and AAH44560). The WWOX cDNA of human and zebrafish have a continuous open reading frame of 1245 bps and 1239 bps encoding a protein of 414 and 412 amino acids with a calculated molecular mass of 46.68 kD and 37.32 kD respectively. The human WWOX gene located at Chromosome 16q23.3-q24.1, which contained two WW domain and a short chain dehydrogenase/reductase (SDR) domain. In human, the WWOX is widely distributed in brain, lung, liver, spleen, kidney, and prostate gland. It may involved in the protein-protein interaction and the steroid metabolism. However, the physiological function of WWOX still need to be further investigation.