Celebrex減低缺氧-回流所引起的骨骼肌損傷機轉之研究

止血帶 (Tourniquet)的施行有助於手術的進行，但也因此會引起缺血/再灌流 (Ischemia/Reperfusion, I/R)，而加劇術後的發炎腫脹。Celebrex是一種選擇性抑制環氧酵素-2 (Cyclooxygenase-2, COX-2)的臨床用藥。本研究意欲以大白鼠進行下肢循環停止模擬手術骨骼肌缺血狀態，觀察 Celebrex 抑制 I/R 之發炎作用的能力。結果發現，Celebrex能減少 I/R 下的 IL-1β 量，並且在缺血狀態下所增加的 COX-2 亦能受到抑制。在我們的結果中也發現 P-JNK、P-P38及 PI3K 等蛋白可能參與在 COX-2 蛋白的調節過程中。由此結果，我們發現在骨骼肌遭受 I/R 壓力時，Celebrex可能具有減低 I/R 後發炎作用的能力。

It is necessary to use tourniquet for reducing blood flow under surgical progression although it can cause Ischemia/Reperfusion (I/R) damage to induce inflammation and swelling after surgery. Celebrex is a well-known selective Cyclooxygenase-2 (COX-2) inhibitor used in clinic to treat inflammation. In this study, we aimed to use limb ischemic model in rat to mimic a tourniquet-caused ischemic condition in skeletal muscle, and we tried to observe the ability of celebrex in inhibiting I/R casued inflammation. The results showed that celebrex can reduce I/R caused increase of IL-1β.It was also observed that I/R caused COX-2 protein was reduced as pretreating with celebrex accompanying by the regulation of P-JNK, P-P38 and PI3K. Taken together, we found celebrex possess the ability to reduce I/R caused inflammation in skeletal muscle.