探討奶薊水萃物對腺病毒感染肺細胞的延緩作用

人類腺病毒由Rowe等人在1953年首次由人類腺樣細胞培養（human adenoid cell culture）中分離出來，1956年正式定名為腺病毒（Adenovirus）。在目前約有100種血清型，可感染人類腺病毒目前有51種血清型，6個亞屬（A~F）其中以亞屬B 之血清型7最常造成肺炎等嚴重病症，進而造成病患死亡。腺病毒在國內為嬰幼兒呼吸道感染的第三位，僅次於腸病毒及呼吸道細胞融合病毒。過去的文獻指出，當人類受到腺病毒感染時，血清中的interleukin-6， interleukin- 8，和 tumor necrosis factor alpha（TNF-α）等激素有明顯增加的現象，這些現象說明細胞激素在受到腺病毒感染致病機轉上的重要性。在眾多的植物萃取物中得知奶薊是一種具有抗發炎作用的藥物，故本實驗以In vitro的方式進行實驗。本實驗藉由奶薊水萃物處理A549細胞株再感染腺病毒，藉由細胞病變效應（cytopathic effect；CPE）觀察、細胞計數及細胞激素測定以探討奶薊水萃物是否能有延緩作用。透過CPE觀察、細胞計數初步結果呈現，細胞病變效應及細胞死亡率有減緩及下降的現象。再進一步進行細胞激素測定則與初步結果無直接關聯，必須更深入研究其他細胞激素或蛋白表現作為佐證。

Human adenovirus was first isolated in 1953 by Rowe et al. In 1956, adenovirus from human adenoid cell culture was designated Adenovirus. At present there are about 100 kinds of serotypes, 51 kinds of serotypes of can present human adenovirus infection serotype 7 existing in subspecies B most often cause severe pneumonia and other diseases highly relative to mortality. Following by enterovirus and respiratory cell fusion virus, Adenovirus is the third position of domestic infant respiratory tract infection.In the past, there was a significant increase in the levels of interleukin-6, interleukin-8, and tumor necrosis factor alpha (TNF-α) in the serum when the human was infected with adenovirus. It is important to examine the levels of cytokines in the pathogensis after infected virus.Milk thistle is a medicinal food possessing anti-inflammatory effect. In this experiment, the A549 cell line infected with adenovirus was incubated with milk thistle water extract. The cytopathic effect (CPE), cell count and cytokine assay were conducted to investigate whether the extract had a delayed effect.The results showed that milk thistle water extract can slow down the cytopathic effect and cell death rate. Although inflammatory cytokines did not reveal direct relationship to the previous results, the other cytokines may be possibly involved and needed to be further investigated.