探討台灣兒童過敏疾病與CC16及NOS1基因多型性之關聯性

過敏疾病已成為現代人的文明病之一；根據2005年Y Furuta的文獻，全球兒童過敏疾病的盛行率已提高至40％，所以已成為健康照護中不可輕忽的一部分。近幾年的研究也證實了，除了環境因素會引發之外，過敏疾病與遺傳也有著密不可分的關係。在本研究中，特別探討台灣兒童過敏疾病與CC16和NOS1兩個過敏疾病基因多型性的關連性。我們共收集了233個檢體，設計了過敏疾病家族間基因型的分析、過敏疾病兒童與正常兒童的基因型比較、過敏疾病兒童之各基因型和臨床檢查：血清總IgE、嗜酸性球數目、過敏原特異性IgE及肺功能的關係。在方法上我們收集血液檢體後，萃取DNA，再執行聚合酶連鎖反應 ( PCR )，利用Sau 96 I與PmaC I兩種限制酶，來分析限制片段長度多型性，並加以定序分析及序列的比對，最後以統計方法來分析實驗資料。由實驗結果證實，CC16與NOS1兩個基因的多型性與台灣的國小兒童罹患過敏性疾病是無相關性。CC16與NOS1兩個基因的多型性與臨床血液檢查：血清總IgE值及嗜酸性球數目也是無相關性。

Allergic diseases have become one of the modern diseases of civilization today. According to the 2005 report of Y Furuta, there has been a 40% increase in the number of allergic diseases of children around the world. Recent studies has confirmed that in addition to the environmental factor,genetic have an inseparable relationship. In this study, we discussed the many genetic types of allergic diseases that particularly affect Taiwanese children.We choose two candidate allergic gene CC16 and NOS1, to develop three experiments : to access the genotype among families with allergic disease, to compare the genotype of normal healthy children to allergic disease children, and to analyze the relation between single genotype of allergic disease and clinical serum immunoglobulin E, eosinophil count, specific immunoglobulin E antigen, and lung function examination. We apply the polymerase chain reaction(PCR), restriction fragment length polymorphism(RFLP), and sequencing. According to the results of the experiment, it confirmed there is no relationship between the CC16 and NOS1 gene polymorphism with allergic disease Taiwanese elementary school children.