結合天然物於矽酸鈣複合材料對於硬組織再生的影響

本篇研究在探討天然藥物與矽酸鈣作用在骨細胞使用人體牙髓細胞的複合材料物理化學與生物效應。矽酸鈣是骨傳導及生物活性物質，檜木純是一種天然材料，它具有抗菌和消炎作用。續斷已被用於傳統中國醫學數百年其作用為抗骨質疏鬆，滋補和抗老化劑，腰背痛，外傷性血腫的治療，預兆性流產和骨折。對於這項研究我們結合了矽酸鈣與不同比例的檜木純及續斷準備做出新的生物活性及生物可降解性骨修復材料。本研究為復合材料形成在前後浸泡模擬體液後的類骨磷灰石、徑力拉伸強度、釋放離子、重量損失的驗證。另外，我們還進行檜木純及續斷與矽酸鈣複合材料對於體外人體牙髓細胞（hDPCs）釋放的影響及其行為。結果顯示含有檜木純的矽酸鈣複合材料具有較好的抗菌消炎能力和細胞生物相容性，對於固化時間及人體牙髓細胞的成骨能力都也顯著的表現，在含有續斷的矽酸鈣複合材料在浸沒在模擬體液中後，微結構圖像顯示覆蓋在矽酸鈣 /續斷複合材料緻密骨狀磷灰石層。在體外細胞實驗表明，豐富的續斷複合材料可以促進人牙髓細胞的增殖和分化。然而，當在複合物中的續斷的量超過5％，在我們的研究中，檜醇結合矽酸鈣複合材料不僅抑制炎性細胞因子的表達水平，同時也有更好的相容性，抗菌性和人體牙髓細胞的成骨細胞分化的能力。矽酸鈣的複合材料中釋放的續斷會刺激人體牙髓細胞的細胞數量和成骨蛋白。矽酸鈣的骨生成結合續斷的材料，給了一個強而有力的理由相信這些鈣基複合水泥可能證明是有前途的骨修復材料。

This study investigates the physicochemical and biological effects of natural medicines on the calcium silicate (CS) composite of bone cells using human dental pulp cell. CS is an osteoconductive and bioactive material. Hinokitiol is a natural material and it has antibacterial and anti-inflammatory effects. Xu Duan (XD) has been used in Traditional Chinese Medicine for hundreds of years as an anti-osteoporosis, tonic and anti-aging agent for the therapy of low back pain, traumatic hematoma, threatened abortion and bone fractures. For this research we have a series of CS composites with different ratios of Hinokitiol and XD were prepared to make new bioactive and biodegradable biocomposites for bone repair. Formation of bone-like apatite, the diametral tensile strength, and weight loss of composites were considered before and after immersion in simulated body fluid (SBF). In addition, we also examined the effects of Hinokitiol and XD released from CS composites and in vitro human dental pulp cell (hDPCs) and studied its behavior. The results show the hinokitiol-modified on CS cements had better antibacterial and anti-inflammatory abilities and cytocompatibility than CS cements. Otherwise, the hinokitiol-modified CS cements had suitable setting times and better odontoblastic potential of hDPCs. The XD on CS composite after immersion in SBF, the microstructure image showed a dense bone-like apatite layer covered on the CS/XD composites. In vitro cell experiments shows that the XD- rich composites promote human dental pulp cells (hDPCs) proliferation and differentiation. However, when the XD quantity in the composite is more than 5 %, the amount of cells and osteogenesis protein of hDPCs were stimulated by XD released from CS composites. In our study, hinokitiol-modified CS cements not only inhibited the expression level of inflammatory cytokines, but also had better cytocompatibility, antimicrobial properties and active ability of odontoblastic differentiation of hDPCs. The combination of XD in osteogenesis of CS gives strong reason to believe that these calcium-based composite cements may prove to be promising bone repair materials.