建構和評估Gellan gum/ Graphene薄膜作為傷口敷料之可行性

本實驗利用結冷膠(Gellan gum、GG)混合石墨烯(Graphene、GO)研製出一種新的具有可降解性、保濕性的傷口敷料，促進傷口癒合。結冷膠是一種陰離子多醣體，具有獨特的生物相容性和價格便宜的特性。石墨烯是一種碳原子的單層薄片，具有獨特的熱、機械性質和電子的特性。結冷膠與石墨烯混合採兩種方式進行，一是將結冷膠和石墨烯混合製膜，二是將石墨烯散佈在結冷膠膜表面，經過交聯作用後而製成GGM和GGS薄膜。薄膜經交聯後，在含膠量和含水量的實驗結果顯示，石墨烯存在不影響交聯結果，GGM的含膠量為63.97%，含水量為94.27%；GGS的含膠量為60.82%，含水量為95.72%。在抗菌實驗的結果顯示，添加石墨烯於結冷膠表面或是混合成薄膜，對於傷口的抗菌效果並不明顯。由動物實驗結果顯示，GGM、GGS薄膜，能夠有效的促進傷口癒合，GGM在術後3、7、14天，傷口癒合程度達25.85%、45.18%、88.98%；GGS在術後3、7、14天，傷口癒合程度達20.85%、36.88%、75.38%，並且改善敷料更換時對於傷口二次傷害的缺點。

In this study we prepared thick films of Gellan gum (GG)/ Graphene (GO) for wound-dressing applications. Gellan gum, an anion polysaccharide, it has biocompatibility and low production cost. Graphene, a single-atom-thick of carbon atoms, it possesses thermal, mechanical, and electrical properties. The applied film was fabricated using two different methods. The first method used of gellan gum mixed with graphene and reacted with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) to obtain a cross-linked film (GGM). The second method that of the prepared graphene is spray on gellan gum film, and then through EDC cross-linking to form the GGS film. The GGM with 63.97% gel content and 94.27% water content. The GGS with 60.82% gel content and 95.72% water content. Antimicrobial susceptibility test has resulted that GGM and GGS without effective antimicrobials for prevention and treatment of infections. Animal wound healing study, on day3, 7, 14, after surgery, the wound size reduction (%) in the GGM-treated group was 25.85%、45.18%、88.98%. On day3, 7, 14, after surgery, the wound size reduction (%) in the GGS-treated group was 20.85%、36.88%、75.38%. Thus, the GGM, GGS films developed in this study has potential for future use in surgical applications.