順鉑/碳奈米點混合物在結腸癌細胞中的抗腫瘤活性

通過一鍋微波合成光致發光碳奈米點(CDs)。該CDs已有報導低成本和生物相容性。在本研究中，研究了一種新型混合物—順鉑/碳點(Cisplatin/CDs)對結腸癌細胞的抗腫瘤活性。由於CDs的親水性和帶電改善了Cisplatin進入細胞的問題，並且增強了對體外結腸癌細胞的功效。將Cisplatin作為MTT測定中的對照組，用於評估Cisplatin/CDs複合物的抗癌活性。當Cisplatin/CDs濃度高於30 μM時，複合物比單獨使用Cisplatin有更好的抗腫瘤活性。然而，進一步發現CDs對癌細胞和正常細胞均有毒性，測量CDs的LD50約為600 μg/ mL。將CDs當用作生物成像中的螢光團時，CDs對細胞可能不是完全無毒。

Photoluminescent carbon nanodots (CDs) were first prepared by a green and one-pot microwave synthesis. The CDs have been reported low-cost and bio-compatible. In this study the antitumor activities of a novel class of mixed Cisplatin and CDs against colon cancer cells were investigated. The hydrophilic and charged CDs improved the complexing of Cisplatin with them and enhanced the efficacy against colon cancer cells in vitro. Cisplatin was taken as a control sample in the MTT assay which was used to evaluate the anticancer activity of Cisplatin/CDs complex. When the concentration of Cisplatin/CDs was above 30 μM, the complex showed a better antitumor activity than Cisplatin alone. However, it was further found that not only Cisplatin but also the CDs were toxic to both cancer and normal cells. The measured LD50 of CDs is approximately 600 μg/mL. The result shows that CDs may not be considered fully non-toxic to cells when used as a fluorophore in bio-imaging.