神秘果乙醇萃取物的抗氧化功效及對C2C12細胞攝入葡萄糖之影響

神秘果(Synsepalum dulificum)的漿果具有特殊味覺修飾功能。近年的動物實驗發現，神秘果粉末具有降低胰島素阻抗性、增加胰島素分泌的功能。為了確認神秘果中的有效成分，本實驗將神秘果分為全果、果皮+果肉、種子三部位，利用95%乙醇進行萃取，測試神秘果三部位之抗氧化作用，並利用C2C12細胞進行2-NBDG的攝入實驗，確認神秘果各部位對C2C12細胞葡萄糖攝入量之影響。在多種抗氧化效果指標測試下，以果皮+果肉的抗氧化效果最佳，可能與果皮+果肉含大量酚類及類黃酮類物質相關。在葡萄糖攝入量實驗中，使用神秘果三部位萃取物培養30分鐘，發現三個部位的乙醇萃取物皆有刺激2-NBDG攝入的功能。全果乙醇萃取物在低濃度(50μg/ml) 時、果皮+果肉乙醇萃取物在高濃度(700μg/ml)時、種子乙醇萃取物則在低濃度(20 μg/ml)時，均與無添加乙醇萃取物的組別相比有顯著差異，其中以種子乙醇萃取物處理的敏感度最佳。若使用神秘果乙醇萃取物進行12小時預處理實驗，則全果、果皮+果肉、種子乙醇萃取物皆出現刺激C2C12細胞株攝入2-NBDG的效果，且在添加胰島素的組別，以種子乙醇萃取物刺激葡萄糖攝入的效果最好，且PI3K活性與未添加乙醇萃取物組別相比，皆達到顯著差異。因此，本實驗推測神秘果萃取物可透過PI3K路徑刺激C2C12細胞攝入葡萄糖，達到降低血糖的效果。

Miracle Fruit (Synsepalum dulificum) is known for its unique taste modifying properties. Animal studies show that miracle fruit has effect on insulin resistance induced by fructose-rich chow in rats. The aim of this study was the antioxidant effects and investigate the effect on the ethanolic extracts of miracle fruit (whole fruit, peel+pulp and seed ) on 2-NBDG uptake and PI3K activities by C2C2 cell line. We found that peel+pulp has good antioxidant effects. In treat 30 min experiment, whole fruit ethanolic extract at 50 μg/ml, peel+pulp ethanolic extract at 700 μg, seed ethanolic extract at 20 μg/ml has significant difference. In the pre-treat 12 hours experiment, the ethanolic extraction of whole fruit, peel + pulp and seeds had significantly effect at any concentration. There ware significant difference in PI3K activity experiment. This indicated that the ethanolic extract of miracle fruit activated the glucose uptake by PI3K pathway.