蘋果多酚改善在卵巢切除之大白鼠引起的骨質疏鬆

骨質疏鬆症所造成的嚴重併發症，正逐步的威脅人類的健康，同時也造成社會沉重的負擔。為避免藥物價格昂貴及副作用，發展以代替藥物的營養策略，來優化骨骼健康。先前文獻指出，蘋果多酚具有抗過敏、抗氧化、抗癌、預防心血管疾病以及美白功效。因此本實驗利用蘋果多酚，是否也能改善骨質疏鬆症。經由建立大鼠卵巢摘除術，來模擬停經後婦女缺乏雌激素引起骨質疏鬆的動物模式，並與假手術的大鼠比較，每日依飲食量給予 0.1%、0.5 及 1% 蘋果多酚餵食8週。實驗結果顯示，經由血液分析，蘋果多酚可以有效降低卵巢摘除鼠ALP 濃度的上升; 在組織免疫病理學檢測方面，發現卵巢摘除鼠股骨骨小樑中密度顯著減少，脂肪細胞生成增加，給予蘋果多酚可改善此情況 ; 而且蘋果多酚有效提升卵巢摘除引起之 Runx2表現量減少及降低第一型血色素氧化酵素(HO-1) 表現量上升之現象 ; 可抑制RANK 和RANKL 的表達。綜合以上實驗的結果顯示，蘋果多酚可以改善卵巢摘除所造成大鼠骨質的流失。所以蘋果多酚應具有開發成之保健食品潛力，用來預防停經症候群併發的骨質疏鬆症。

Osteoporosis leads to serious complications, is a gradual threat to human health and causes social burden. Due to the anti-osteoporotic drug being expensive and more side effects, the development of nutrition strategy to replace the medicine is suggested to optimize the skeleton health. The previous literatures pointed out that the apple polyphenol possessed the anti-allergy, the antioxidant property, the anti-cancer, cardiovascular disease prevention as well as the flesh whitening effect. Therefore, we assessed the apple polyphenol whether also can improve osteoporosis. By way of the establishment of ovariectomy-induced bone loss in rat mimicked the osteoprosis in menopausal woman, and compared with sham-operated rats. Either a control diet or diet supplemented with 0.1%, 0.5% and 1% apple polyphenol was applied for 8 weeks before necropsy. The experimental results showed that apple polyphenol can reduce the level of ALP, which always risen in ovariectomic rats. In the immunohistochemistry examination, the trabecular bone was decreased in density and adipose cell was significant increased in femoral bone of ovariectomic rat. Giving the apple polyphenol would improve this situation. Additionally, the decreased expression of Runx2 and high level of heme oxygenase-1 (HO-1) in ovariectomic rat were also improved as treated with apple polyphenol. Apple polyphenol also suppressed the expression of RANK and RANKL in ovariectomic rat. Based on the above experimental results show that apple polyphenol should have the potentials to develop a functional food in menopause induced osteoporosis.