Hedgehog在卵巢之表現與卵細胞成熟度相關性分析

Hedgehog路徑在果蠅性腺中發揮重要作用，調控細胞增殖分化，但是在哺乳類性腺中的作用上未確實。為了確認Hedgehog路徑在卵巢、輸卵管和子宮的表達和變化，利用組織免疫染色和西方墨點法進行分析。卵細胞成熟過程，Hh蛋白的分泌不是卵細胞自行產生，而是屬於旁分泌，由顆粒細胞所產生。FSH的Hh之間的關係是負向的的，隨著PMSG的提高Hh的表現量也會跟著降低，在卵泡成熟可能是因為隨著身理週期FSH的減少促使Hh路徑的表現使卵泡成熟。卵巢成熟過程，隨者FSH的表現限增加，而去抑制Hh路徑表達量降低，卵巢成熟過程Hh扮演了一定的中要角色。輸卵管在FSH影響下，Hh路徑表現量是增加的，這很有可能代表著輸卵管在為了卵細胞和精蟲準備一個適當的受精環境，當Hh表現量下降時有可能去影響到精卵結合的過程。子宮上段Hh是由基質細胞分泌出Hh，幫助上皮細胞的生長使子宮內膜增生。Hh路徑不管是在卵泡、卵巢、輸卵管還是子宮都有一定的重要性，可做為將來治療不孕症的一個重要的指標。

The Hedgehog pathway plays an important role in Drosophila gonads, regulating cell proliferation and differentiation, but not in mammary gland. In order to confirm the expression and changes of the Hedgehog pathway in the ovaries, fallopian tubes and uterus, analysis was performed using tissue immunostaining and Western blotting. oocyte maturation process, Hh protein secretion is not self-produced oocyte, but belong to paracrine, produced by the granulosa cells. The relationship between the Hh of FSH is negative, and the expression of Hh will decrease with the increase of PMSG. The follicle maturation may be due to the decrease of follicular maturation due to the decrease of HH pathway with the decrease of PEH. Ovarian maturation process, with the increase in the performance limit of FSH, and to inhibit the Hh path expression decreased, ovarian maturation process Hh played a certain role in the play. In the fallopian tube under the influence of FSH, Hh path performance is increased, which is likely to represent the fallopian tube in order to egg cells and sperm to prepare a suitable fertilization environment, when the Hh expression decreased may affect the process of sperm and egg. The upper part of the uterus Hh is secreted by the stromal cells Hh, to help epithelial cells to grow to endometrial hyperplasia. Hh pathways, whether in follicles, ovaries, fallopian tubes or uterus have a certain importance, can be used as an important indicator of future treatment of infertility.