長鏈非編碼核糖核酸其基因變異與糖尿病視網膜病變臨床表現的相關性

糖尿病在全世界的盛行率不斷增加，糖尿病視網膜病變是糖尿病患者常見的併發症，其中危險因子包括高血糖、高血壓、腎臟病、糖尿病罹病時間、高血脂及基因等，而許多長鏈非編碼核糖核酸也已經被證實在糖尿病視網膜病變扮演重要角色，本研究主旨在探討是否 LINC00673的單一核?酸多型性會影響糖尿病視網膜病變的臨床表現。本研究採取病例對照研究方式，本篇研究共計730名受試者，其中276位有糖尿病視網膜病變及454位沒有糖尿病視網膜病變，收錄所有受試者的臨床資料及血液。收錄之血液使用即時聚合?連鎖反應(real-time polymerase chain reaction, real-time PCR)分析定量LINC00673的三個單一核?酸多型性位點 (rs6501551, rs9914618, rs11655237)。研究結果顯示在非增殖性糖尿病視網膜病變亞組相較於沒有糖尿病視網膜病變組有較高的機率有LINC00673 rs11655237 CT 基因型 [adjusted odds ratio (AOR): 1.592, 95% confidence interval (CI): 1.059–2.395, P = 0.026]、 CT+TT 基因型 (AOR: 1.255, 95% CI: 1.029–1.531, P = 0.025) 和T 等位基因(AOR: 1.185, 95% CI: 1.004–1.397, P = 0.044) 。另外在有糖尿病視網膜病變組中， LINC00673 rs11655237 CT+TT 基因型的患者會較LINC00673 rs11655237 CC 基因型的患者有較短的糖尿病罹病時間 (10.44 ± 7.10 versus 12.98 ± 8.34年, P = 0.009)。總結來說，有LINC00673 rs11655237 T等位基因的糖尿病病人有較高的可能性產生非增殖性糖尿病視網膜病變，而有LINC00673 rs11655237 CT+TT基因型的糖尿病視網膜病變病人會在較短的時間產生糖尿病視網膜病變。因此建議所有糖尿病病人應該做基因篩檢，若是含有以上基因型應該更加積極控制血糖。

The prevalence of diabetes mellitus (DM) is increasing all over the world. Diabetic retinopathy (DR) is a common complication of DM. The risk factors of DR include hyperglycemia, hypertension, renal dysfunction, the duration of DM, hyperlipidemia, genetics, etc. The association of many different long non-coding RNA (lncRNA) and DR has been confirmed. The objective of this study is to evaluate the possible correlation between the single nucleotide polymorphisms (SNP) of LINC00673 and the clinical characteristics of DR. A total of three loci of LINC00673 SNPs (rs6501551, rs9914618, and rs11655237) were genotyped using TaqMan allelic discrimination in 276 patients with DR and 454 patients without DR. The results showed that LINC00673 SNP rs11655237 CT genotype (AOR: 1.592, 95% CI:1.059–2.395, p = 0.026), CT + TT genotype (AOR: 1.255, 95% CI: 1.029–1.531, p =0.025), and allele T (AOR: 1.185, 95% CI: 1.004–1.397, p = 0.044) revealed higher ratios in the non-proliferative diabetic retinopathy (NPDR) subgroup than in the non-DR group. The LINC00673 SNP rs11655237 CT + TT variant showed significantly shorter interval of DM than the LINC00673 SNP rs11655237 CC genotype (10.44 ± 7.10 vs. 12.98 ± 8.34 years, p = 0.009) in DR group. The conclusions of the study are the LINC00673 SNP rs11655237 T allele is associated with a higher probability of NPDR development and the patients with the LINC00673 SNP rs11655237 CT + TT variant exhibited a shorter DM interval. Consequently, genetic screening might be suggested for patients with DM. Patients with these LINC00673 genetic polymorphism should observe stricter blood sugar control.