介白素-1β3954基因多樣性與大腸癌之關聯

大腸直腸癌為全世界第三大常見的惡性腫瘤。曾有文獻提出可能是導致大腸直腸癌發生的危險因子包含年齡增加、性別 (男性較易罹患大腸直腸癌)、發炎性腸道疾病、一些遺傳條件、家族史。IL-β擁有可以增強發炎細胞從血液移動到發炎組織，調控細胞外基質降解，來調解發炎反應的多效性生物功能。之前有研究指出單核酸多型性與癌症和多種疾病有關。而本實驗目的為研究IL-β (+3954) 單核甘酸多型性與台灣人口大腸直腸癌的相關性。利用聚合酶連鎖反應-限制酶切割片段長度多型性的方法來分析74位大腸直腸癌患者和72位未得過大腸直腸癌的對照組，其IL-β (+3954) 基因多型性等位基因頻率的關聯性。結果顯示IL-β (+3954) 基因多型性在大腸癌發病和對照組間有顯著差異性 ( P < 0.001 )。另外，在大腸直腸癌實驗組中，IL-β (+3954) 同型合子T/T等位基因與CT+CC基因型頻率與腫瘤分期 ( P = 0.232 ) 、腫瘤大小 ( P = 0.507 ) ，近端淋巴轉移 ( P = 0.142 ) ，遠端轉移 ( P = 0.265 )，均無顯著差異性。我們的研究首先提出在台灣地區人群中IL-β (+3954) 基因多型性與大腸直腸癌發生是有相關的。

Colorectal cancer (CRC) is the third most common malignancy worldwide. It has been proposed that risk factors for colorectal cancer include increased age, gender (men are more predisposed to the development of colorectal cancer), the presence of inflammatory bowel disease, certain hereditary conditions and a family history of colorectal cancer. The interleukin1β (IL-1β) protein has many pleiotropic biological functions including the mediation of inflammation by enhancing the movement of inflammatory cells from the blood to inflamed tissues and the regulation of extracellular matrix degradation. Reports indicate that single nucleotide polymorphism (SNP) is associated with cancers and diseases. We studied the association of the IL-1β-3954 C/T polymorphism and CRC in Taiwan population. Using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), the allele frequency of the IL-1β polymorphism was investigated in 74 patients with colorectal cancer and 72 control. It shows that polymorphism of IL-1β-3954 in between CRC onset patients and controls are significant difference (p<0.001). In addition, the frequencies of IL-1β-3954 homozygous T/T allele and C/T+C/C genotype in CRC group was not associated with stage (p=0.232)、tumor size (p=0.507)、 lymph node metastasis (p=0.142)、distant metastasis (p=0.265). Our study presents preliminary but intriguing data suggesting that IL-1β3954 may be associated with CRC onset in Taiwan region.