利用Phage Display技術找出與阿滋海默症作用之胜肽

Alzheimer's Disease 是一種神經退行性疾病，而且會隨著年紀的增加變得更嚴重直至死亡，此種疾病會使得患者記憶力衰退、執行能力變差、說話寫作判斷能力下降等等諸多的生理病況。我們對於篩選Alzheimer's Disease 有親和力作用的胜肽序列感到很大的興趣，希望在將來能夠發展成為藥物。本實驗的目的為使用phage display C-7-C的方式找出能夠與APP結合的胜肽。我們會先表現GST-C terminal APP和GST 控制組的蛋白質，將噬菌體胜肽庫與與純化的蛋白質做反應，並將未結合的噬菌體洗掉再萃取出有結合的噬菌體，得到這些有反應結合的噬菌體胜肽序列就可以進行DNA定序。在進行3～4的反應和定序並經過Translate tools網站的基因編碼比對，我們比對雖然沒有得到極高的重複性，但是我們找到的這些peptides可能都是與Alzheimer's Disease有相關的，以後我們還是可以拿這些peptides去做與Alzheimer's Disease有關的細胞、藥物、動物實驗，也可以修正改進phage display 實驗的過程並再次送去做DNA定序找到與APP結合反應的胜肽，並利用找到重複相關性更高的胜肽去做更進一步的細胞、抗體、藥物等等實驗。我們使用phage display 的方法總共得到了9個與APP可能有關的peptides。

Alzheimer’s disease is a type of progressive disease associated with memory loss, difficulty in decision making, lowered speech and writing capabilities and many other physiological conditions, and may ultimately lead to death with age. We are very interested in screening peptide sequences to analyze their affinity for Alzheimer's Disease, in hopes of finding results that can be evaluated and used for developing drugs to combat the disease in the future.The purpose of this experiment is to find peptides that can bind to APP by using phage display C-7-C. We first displayed the proteins of the GST-C terminal APP and GST control group, allowed the phage peptide library to react with the purified protein, then washed off the unbound phage and extracted the bound phage, which was then used for DNA sequencing. After performing 3~4 reactions and sequencing and comparing them with the bioinformatics system workbench, we did not get very high repeatability, but the peptides we found appeared to be connected to Alzheimer's Disease.In the future, we can still use these peptides to carry out cell, drug, and animal experiments on Alzheimer's Disease. By modifying and improving upon the phage display experiment, we can send the extracted phage peptide again for DNA sequencing to find peptides that bind to APP, and use the results with high repeatability to set up further experimentation with cells, antibodies, drugs, etc. The phage display method we used allowed us to obtain a total of 9 peptides that may be related to APP, but because repeatability was not high, more experiments are needed to find peptides with a higher affinity to APP.