Isolation and identification of steroidal saponins in Taiwanese Rhizoma Paridis cultivar (Paris formosana Hayata)

Nine steroidal saponins were found in Taiwanese Rhizoma Paridis cultivar (Paris formosana Hayata). They were isolated through liquid-liquid partition and high performance liquid chromatography (HPLC), and identifed by NMR and HPLC-Mass. The nine saponins included three furostanol glycosides: 26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamnopyranosyl- (1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside, 26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-arabinofuranosyl- (1→4)]-β-D-glucopyranoside and 26-O-β-D-glucopyranosyl-22α- methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosy- (1→2)-O-[β-D-glucopyranosyl (1→3)]-β-D-glucopyranoside, two pennogenyl glycosides (spirostanol glycosides): (25R)-spirost-5-en-3β, 17-diol 3-O-α-L-rhamnopyranosy-(1→4)-O-α-L-rhamnopyranosyl- (1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside and (25R)-spirost-5-en-3β, 17-diol 3-O-α-L-rhamnopyranosyl-(1→2)- [α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside, and four diosgenyl glycosides (spirostanol glycosides): (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamnopyranosyl-(1→4) -O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside, (25R)-spirost-5-en-3β-ol (25R)-spirost-5-en-3β-ol 3-O-α-L- rhamnopyranosyl-(1→2)-[β-D-glucopyranoside-(1→3)] -β-D-glucopyranoside, 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L- arabinofuranosyl-(1→4)]-β-D-glucopyranoside and (25R)-spirost-5-en- 3β-ol 3-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-glucopyranoside.