綠梔子素交聯聚乙烯醇/明膠/竹炭奈米顆粒之複合水凝膠於生物醫學上之應用

聚乙烯醇和明膠都具有良好親水性、生物相容性和生物降解性，這些材料被廣泛應用於生醫領域，如藥物載體、組織工程支架、傷口敷料等。竹炭具有良好吸附性和富有礦物質並且可溶於水，綠梔子素具有低的生物毒性是戊二醛的一萬分之一，同時能和明膠、幾丁聚醣等含有胺基高分子聚合物產生交聯反應。在本研究中將不同比例的聚乙烯醇和明膠進行混合並比較其降解度和膨潤度和拉伸性質，最終篩選出本研究組別作為聚乙烯醇/明膠複合膜材組別，此外，還添加不同重量比竹炭奈米顆粒和不同濃度綠梔子素藉此探討對於複合膜材的性質影響。結果顯示添加竹炭奈米顆粒和綠梔子素都能顯著提升聚乙烯醇/明膠複合膜材抗降解性和拉伸強度。在生物相容試驗七十二小時內都呈現良好的生物相容性，並且綠梔子素交聯的膜材不論在拉伸強度、抗降解性和生物相容性都比添加竹炭奈米顆粒膜材還要高。最終本研究採用1%重量比竹炭奈米顆粒和0.05%綠梔子素同時添加到複合膜材中，結果在抗降解性試驗發現浸泡磷酸鹽緩衝溶液第七天能夠顯著提升，然而拉伸強度與0.05%綠梔子素沒有顯著差異。

Polyvinyl alcohol (PVA) has good hydrophilicity, chemical stability and biocompatibility, while gelatin(Gel) has good biocompatibility and good biodegradability. These materials are widely application for biomedicine such as drug carriers, scaffolds of tissue engineering, wounds dressing, etc. Bamboo charcoal nanoparticles (BCNPs) has good adsorption and mineral richness and is soluble in water. Genipin (GP) was about 10000 times less cytotoxic than glutaraldehyde. also can cross-linking for amine-based polymers such as gelatin and chitosan. In this study, mixed different weight ratios of polyvinyl alcohol and gelatin and compared their degradation degree, swelling degree and mechanical properties. Finally, the research group was selected as the polyvinyl alcohol/gelatin composite film group. In addition, different weight ratios of BCNP and different concentrations of GP were added to nanocomposite film. Explore the effect on the properties of the composite film. The results showed that the addition of BCNP and GP can significantly improve the degradation resistance and tensile strength of the PVA/Gel composite film. In biocompatibility test all film group show good cell viability in 72 hours. Genipin cross-linked film is stronger than the BCNP in terms of tensile strength, degradation resistance and biocompatibility. Finally, in this study, 1% BCNP and 0.05% GP were added to the composite film at the same time. As a result, it was found in the anti-degradation test that the soaked phosphate buffer solution could be significantly improved on the 7 day, but the tensile The intensity was not significantly different from the 0.05% GP solution.