冰醋酸作為溶劑對油酸調節之聚己內酯/氫氧基磷灰石奈米複合膜材特性和生物活性之影響

聚己內酯具有良好的生物相容性、生物降解性及優良的機械性質，常與具骨導引性之氫氧基磷灰石結合，應用於組織工程支架的製備。高分子支架的製備常使用氯仿、二氯甲烷作為溶劑，以上物質屬於列管毒性化學物質，對人體有危害性，另一方面，有機溶劑容易誘導膜材中氫氧基磷灰石粒子產生凝集而降低膜材機械性質。因此，本研究運用溶劑鑄造法結合油酸的添加所製備PCL-OA-HA複合膜材，探討溶劑改變對膜材性質的影響，結果發現以醋酸為溶劑所製備的PCL-OA-HA(HAc)複合膜材與以1,4 dioxane為溶劑所製備的PCL-OA-HA(1,4-dioxane)複合膜材的的表面性質相似，但可以觀察到表面磷灰石裸露形狀及程度有所差異。觀察浸泡模擬體液七天膜材表面後皆有氫氧基磷灰石礦化層產生，但以PCL-OA-HA(HAc)複合膜材表面產生的氫氧基磷灰石為針狀且礦化層較為均勻。生物相容性評估結果顯示PCL-OA-HA(HAc)複合膜材高於PCL-OA-HA(1,4-dioxane)複合膜材，推論膜材內未揮發完全的溶劑(1,4-dioxane)降低細胞存活率。另一方面，膜材的表面改質可觀察到鹼液濃度及處理時間可增加膜材表面礦化層形成及均勻度。由以上研究結果，本研究以醋酸為溶劑所製備之PCL-OA-HA(HAc)複合膜材能提供良好的生物活性，藉由均勻至密礦化層的產生，可提高硬骨再生與骨整合的能力，未來可做為引導組織再生及硬骨修復方面之應用。

PCL has good biocompatibility, biodegradability and excellent mechanical properties, and combination with hydroxylapatite used in tissue engineering scaffolds produced. Preparation of scaffolds often using chloroform, methylene chloride as a solvent, the above substances are among the toxic chemicals harmful to humans. On the other hand, the organic solvent is easy to induce hydroxylapatite particles agglutination in membrane and mechanical properties of membrane was reduced. Therefore, the present study using solvent casting method combined with oleic acid added prepared PCL-OA-HA membranes, investigate the effect of changing the solvent properties of membranes found acetic acid as a solvent prepared PCL-OA-HA (HAc) similar to the surface properties of the composite membrane prepared with solvent 1,4 dioxane as the PCL-OA-HA (1,4-dioxane) membranes. It can be observed that the shape and extent of surface apatite exposed somewhat differences. After soaking simulated body fluid (SBF) was immersed seven days. PCL-OA-HA (HAc) membrane surface have needle hydroxylapatite layer formation. Biocompatibility assessment showed that PCL-OA-HA (HAc) membrane is higher than the PCL-OA-HA (1,4-dioxane) membranes. membrane inference is not entirely within the volatile solvent (1,4 dioxane) decreased cell survival. Mineralized surface layer membrane formation and evenness and alkali concentration and treatment time related. From the above findings, PCL-OA-HA (HAc) composite membranes can provide a good biological activity, with a uniform layer of mineralization to produce dense, hard bone regeneration and improve osseointegration the ability to guide the future application can be used as a hard bone tissue regeneration and repair that regard.