Assessment of Cell Viability Using the Chronoamperometric Method Based on Screen-Printed Electrodes

This study investigates suitable electrochemical mediators and optimal mediator concentration for breast cancer cells, MDA-MB-231. The optimized mediators were 1mM ferricyanide and 10μM menadione. The specifications for the chronoamperometric detection of ferrocyanide were a linear range of 0.02-0.36mM. The detection limit was 5.0μM and the spiked recovery was 95%-99%. The oxidation currents increased linearly with cultured cell density. Cell viabilities determined using electrochemical methods confirmed those determined by MTT assay. The running time can be reduced to less than 30min. The chronoamperometric method can be used as an alternative method for rapidly assessing the viability of breast cancer cells.