Rooibos萃取物對降血脂功效評估

世界衛生組織已於 1997 年將肥胖明列為一種疾病，同時更將其列為二十一世紀重要的健康議題，而伴隨肥胖症所帶來的血脂異常，在我國所優先採取非藥物的治療對策，因此市面上率續出現預防高血脂相關的健康食品。許多研究中指出天然植物多酚在降血脂及抗肥胖作用上有顯著效果，因此選擇富含多酚的Rooibos萃取粉末(RE)作為研究。首先，測試萃取物對L929和HepG2細胞增生活性，再對0.3mM油酸誘導HepG2油滴堆積及40 % FDC飲食誘導高膽固醇血症的B6小鼠進行降脂功效評估。實驗結果表示，在24小時內並不會對細胞的增生活性造成嚴重的抑制，且在低濃度範圍下(2.68、5.35和16.05 ????g/ ml RE)才對油滴堆積有顯著改善，而在小鼠血清中，1% RE組總膽固醇在第四週及第五週分別下降17 %及15 %，而在LDL-C第四週及第五週分別上升14%和下降2%。綜觀上述實驗結果，因此對於Rooibos萃取物對降血脂功效評估是有效的，故本研究認為Rooibos萃取物具有開發成大眾健康食品的潛力。

The World Health Organization( W.H.O.) listed obesity as a disease in 1997. At the same time,W.H.O. listed obesity as an important health issue in the 21st century. However, dyslipidemia is associated with obesity. Non-drug treatment strategies are given priority in our country, so health foods which are related to the prevention of hyperlipidemia continue to appear on the market. A lot of researches have been pointed out that natural plant polyphenols have significant effects on decreasing blood lipids and anti-obesity, so we choose Rooibos extract (RE) powder, which is rich in polyphenols, for our study on assessement of decreasing blood lipids. First, RE was tested for the cell proliferation of L929 and HepG2, and then HepG2 was induced lipid accumulation by 0.3 mM Oleic acid. B6 mice were induced to hypercholesterolemia by 40% FDC so as to evaluate ability of RE reducing dyslipidemia. The experimental results show that the proliferation of cells will not be severely inhibited in 24 hours, and the accumulation of lipid can be significantly improved at low concentration ranges (2.68, 5.35 and 16.05 ????g/ml RE). In serum of B6 mice, total cholesterol in the 1% RE group decreased by 17% and 15% in the fourth and fifth weeks, respectively, and LDL-C increased by 14% and decreased by 2% in the fourth and fifth weeks. In conclsion, RE is effective in assessing the ability of decreasing lipid accumulation, so this study believes that Rooibos extract has the potential to develop into a public health foods.