蘋果多酚合併維生素D經由調節乙型轉化生長因子延緩糖尿病視網膜病變

糖尿病是常見的代謝性疾病，由糖尿病引起視網膜病變是主要原因之一，視網膜病變分為兩型，非增殖型視網膜病變(NDPR)和增殖型糖尿病視網膜病變(PDR)，早期的持續性慢性發炎，衍生細胞增生和新生血管的變化。過程中的相關因子，如乙型轉化生長因子(Transforming growth factor beta, TGF-β)、超氧岐化酶(Superoxide dismutase, SOD)、蛋白質激酶C(Protein kinase C, PKC)和細胞外訊號調節激酶(Extracellular signal-related kinase, ERK)，對細胞造成的影響，高糖代謝和高氧化壓力的環境下，引起視網膜細胞的損傷，對患者視力產生威脅。實驗中利用抗氧化物蘋果多酚和降血糖血脂的維生素D介入，觀察是否在視網膜病變前期，對細胞之間微環境產生改變，並期待結果中能達到預期的效果。本次研究利用STZ誘導糖尿病，比較藥物二甲雙胍和天然物蘋果多酚與維生素D之間差別。在綜合結果中蘋果多酚和維生素D約能降低TGF-β 70%，PKC 52%，ERK 76%，證實蘋果多酚與維生素D具有抑制發炎因子和血管生成相關因子的效果，且在清除活性氧分子(ROS)的超氧岐化酶(SOD)中發現，蘋果多酚和維生素D平均能增加1919 %的SOD活性，對於視網膜具有保護作用。

Diabetes is a common metabolic disease. Retinopathy caused by diabetes is one kind. Retinopathy is divided into two types, non-proliferative diabetic retinopathy (NDPR) and proliferative diabetic retinopathy (PDR), which are in the early persistent chronic inflammation process related factors, such as transforming growth factor beta (TGF-β), superoxide dismutase (SOD), protein kinase C (PKC) and extracellular signal regulation Kinases (ERK) have an impact on cells. Under the environment of high glucose metabolism and high oxidative stress, they can cause damage to retinal cells and threaten the eyesight of patients. In the experiment, we used antioxidant apple polyphenols and vitamin D to reduce blood sugar and blood lipids to observe whether the microenvironment between cells is changed before and after the retina, and expect the results to achieve the expected effect. In the comprehensive results, apple polyphenols and vitamin D can reduce TGF-β 70%, PKC 52%, and ERK 76%, confirming that apple polyphenols and vitamin D have the effect of inhibiting inflammatory factors and angiogenesis-related factors, and they can eliminate active oxygen. It is found in the superoxide dismutase (SOD) of the molecule (ROS) that apple polyphenols and vitamin D can increase the SOD activity by an average of 1919%, and have a protective effect on the retina.