利用高梁酒粕餵飼蛋雞建立氧化緊迫現象的動物模式

高粱為重要的雜糧作物之一，台灣金門地區以釀酒為主要用途。高粱酒粕為蒸餾高粱酒所產生的廢棄物，富含蛋白質及生理活性成份，過去常提供做為家禽、家畜之飼料，但近年來，畜養多採工廠販售的混合飼料，酒粕的利用逐漸減少。蛋雞所生產的雞蛋不但產量大，成本便宜，而且可連續生產供測試。雞的蛋白質轉譯後修飾與人類相似，所以可利用雞的結果反映在人類in vivo的實驗。高梁酒粕的粗蛋白含20%、澱粉量25%，本實驗想利用高粱酒粕作為蛋雞的飼料配方，藉由飼料的改變建立酯質過氧化的動物模式。36隻蛋雞分為三組，對照A組任食一般飼料，實驗組B、C任食一般飼料其中分別含15%高梁酒粕及30%高梁酒粕，結果發現B組所產雞蛋中蛋黃MDA含量為229.3 nmol/50μl egg yolk，高於正常對照A組的162.9 nmol/50μl egg yolk，經統計發現 (P<0.05)有顯著差異，而C組所產雞蛋中蛋黃MDA含量為289.8 nmol/50μl egg yolk，明顯高於正常對照A組，經統計發現 (P<0.01)有極顯著差異，且隨著高梁酒粕添加比例正相關。綜合以上結果，我們認為在蛋雞的飼料中添加適當比例的高梁酒粕，能夠建立氧化緊迫現象的動物模式。

Sorghum is one of the most important crops all over the world. In Kinmen, the major consumption of sorghum is the liquors. The sorghum distillery residue (SDR) which is obtained from the remnants of the sorthum fermentation and distillation is rich in protein and bioactive components. The SDR contain 20% crude protein and 25% starch.. The purpose of this study was to establish an animal model for testing the oxidative stress induced by feeding sorghum distillery residue in chicken. Thirty-six chickens were randomly divided into 3 groups (n=12), the control group (A group) was fed with basal diets, and other groups were fed with basal diets and 15-30% sorghum distillery residue (B and C groups). The result showed that malondialdehyde (MDA) values in B group (229.3 nmol/50μl egg yolk) (P<0.05) and C group (289.8 nmol/50μl egg yolk) (P<0.01) were significantly higher than that in the control group (162.9nmol/50μl egg yolk). Moreover, the elevation of MDA values in the egg yolk was depended on the increase of sorghum distillery residue in chicken diets. Thus, we suggest that the appropriate sorghum distillery residue supplementation in chicken diet can be suitable for an oxidative stress animal model.