Gellan gum/Glucosamine薄膜包覆口腔癌治療藥物作為治療貼片之研究

口腔癌細胞多發生在舌部，其次為粘膜、牙齦、口部地方。基於此，我們認為如果能開發口腔癌治療貼片，將治療藥物包覆在基材中，直接覆蓋在口腔癌病灶處，可以是一種非侵入性的治療方式，同時具有保護腫瘤切除後傷口。本實驗利用結冷膠 (Gellan gum ; GG) 研製出一種新的具有可降解性且具經濟效益的薄膜，基於文獻回顧我們認為Curcumin (薑黃素; CM) 及 Clioquinol (氯碘仿; CQ) 抗癌機轉可應用於口腔癌治療，研究結果發現 Gellan gum 薄膜具有良好的機械性質，薄膜經交聯後，在含水量和含膠量的實驗結果顯示， CM 及 CQ 的存在下不影響交聯結果， GG-CM 的含水量為 97.6% 及 93% ，含膠量為 87% 及 83.5%；GG-CQ的含水量為 97.6% 及 93.5%，含膠量為 87.3% 及 83.5%。經交聯後的薄膜在 28 天的降解試驗後分別是 GG-CM 91.8% 及 88.6% ； GG-CQ 91.4% 及 90.1%。經交聯後的薄膜在 50 天累積藥物釋放率分別可達到 GG-CM 89.6% 及 86.5% ；GG-CQ 86.8% 及 82.4%。動物模型建立方面，經由 14 週的 DMBA 誘導，結果顯示，可見頰囊外觀具有明顯的表皮變異，除外觀可見到局部脫毛，且有出血、潰瘍及紅斑病變現象，亦可見到顆腫瘤生成。

Oral cancer frequently happens at tongue, mucous, gingiva and oral area. Base on this, we consider that developing the patch containing the drug as one of the basic materials and covering on the affected region of the oral cancer can be a non-invasive treatment and also protect the wound that causes by the tumor-cutting operation. We used the Gellan gum to develop a new patch which has degradability and also economic in our experiment. According to the previous, Curcumin and Clioquinol can be used to apply on the treatment of the oral cancer. We have found that Gellan gum gets the fine mechanical property in the result. And after the cross-linking process, the experimental result of gel content and water content showed that the existence of CM and CQ don''t affect the cross-linking consequence. The water content of GG-CM are 97.6% and 93% and the gel content are 87% and 83.5%. The water content of GG-CQ are 97.6% and 93.5% and the gel content are 87.3% and 83.5%. The cross-linked patch showed the GG-CM 91.8% and 88.6%,GG-CQ 91.4% and 90.1% in the degradability test after 28 days. The cross-linked patch showed the GG-CM 89.6% and 86.5%, GG-CQ 96.6% and 82.4% in the drug accumulative release test after 50 days. In the animal model, used DMBA induced after 14 weeks, the results showed that the appearance of the cheek pouch has a significant variation in the epidermis, the appearance can be seen localized hair removal, and bleeding, ulcers, lesions of erythema and tumorigenesis.