人類微小病毒B19殼體蛋白獨立區域抗體對Balb/c小鼠心臟影響之研究

許多文獻報導在心臟疾病患者的血液或心臟組織中有B19病毒的蹤影，並且發現感染B19病毒與心肌炎之間有著相關性。先前實驗室發現B19-VP1u IgG抗體可促進NZB/W F1小鼠心臟發炎情形產生。因此本研究將進一步探討B19-VP1u IgG抗體及不同片段B19-VP1u蛋白質之IgG抗體對於Balb/c小鼠心臟之影響。結果發現相較於正常Balb/c小鼠對照組，施打B19-VP1u IgG抗體會促進Balb/c小鼠左心室心臟基質金屬蛋白酶MMP9活性，並且透過NF-κB 蛋白訊息傳遞路徑。另外，組織病理切片中也發現施打B19-VP1u IgG或不同片段B19-VP1u蛋白質之IgG的Balb/c小鼠左心室心臟有明顯免疫細胞浸潤的現象。綜合以上研究結果顯示施打B19-VP1u IgG抗體確實會造成Balb/c小鼠心臟發炎情形產生。

Many literatures have reported the presence of the B19 virus in the blood or heart tissue of patients with heart disease, and found a correlation between the infection of B19 virus and myocarditis. Previous studies have demonstrated that B19-VP1u IgG antibody could stimulate myocarditis in NZB/W F1 mice. Therefore, in the present study, we further investigate the effect of B19-VP1u IgG and IgG B19-VP1u fragments on the heart of Balb/c mice. The results showed that compared with the normal Balb/c mouse control group, the injection of B19-VP1u IgG antibody promoted the activity of matrix metalloproteinase MMP9 in the left ventricle of Balb/c mice, which is through NF-κB proteins. However, the expression of myocardial infarction protein CK-MB showed no significant difference in the control group. On the other hand, histopathological sections also showed significant immune cell infiltration in the left ventricular heart of Balb/c mice in the B19-VP1u IgG or IgG B19-VP1u fragments groups. Based on the above results, it was shown that B19-VP1u IgG may cause heart inflammation in Balb/c mice.