人類紅血球細胞膜和磷脂膽鹼微脂粒系統中分配係數對於七種酚類試劑各別以及組合後抗氧化效果的重要性

中文摘要 在人類紅血球細胞膜及微脂粒系統中以Fe2+誘發脂質過氧化，比較七種酚類(兩種酚酸caffeic acid，gallic acid和五種黃酮物質，catechin，epicatechin，quercetin，rutin，myricetin)以及與-tocopherol，-carotene，vitamin C交互作用的抗氧化活性，五個濃度為10 mol/L的黃酮類在兩系統的抗氧化順序為catechin > epicatechin > rutin > quercetin > myricetin (p<0.05)，此與分配係數的順序完全相反。測量七個酚類化合物分別與-tocopherol，-carotene，vitamin C交互作用對於Fe2+誘發脂質過氧化的抑制，5 mol/L -tocopherol 加上 5 mol/L catechin，caffeic acid 或epicatechin 的組合會產生協同效果，而5 mol/L vitamin C 與5 mol/L 的各酚類抗氧化劑亦相同，分配係數對於七個酚類試劑與其他抗氧化劑的交互作用扮演著重要角色。caffeic acid，catechin 和epicatechin 與-tocopherol，-carotene，vitamin C組合都顯著增強對脂質氧化的抑制能力，此物質可能對於氧化相關疾病的預防有所幫助。

Abstract Antioxidant activities of seven phenolic agents against Fe2+-induced lipid oxidation were compared with -tocopherol, -carotene, vitamin C in human erythrocyte membrane ghosts and liposome systems. The antioxidant activity of five test flavonoids followed the order catechin > epicatechin > rutin > quercetin > myricetin in both systems (p<0.05), which was negatively correlated with their partition coefficients. The antioxidant interaction of these phenolic agents with -tocopherol, -carotene or vitamin C in inhibiting Fe2+-induced lipid oxidation was examined. Synergistic effects were present in the combinations of -tocopherol plus caffeic acid, catechin or epicatechin as well as all combinations of vitamin C plus phenolic antioxidants. Based on the stronger individual and combined effects present in caffeic acid, catechin and epicatechin, the application of these three phenolic agents with or without -tocopherol, -carotene, vitamin C may provide stronger protective benefits against lipid oxidation, which may be helpful for oxidation-related diseases prevention.