苯基汞影響成熟雄性小白鼠之運動行為及其他生理功能的研究

在成熟雄性小白鼠單一皮下注射苯基汞 (PMA) ，給予濃度分別是：7.5、15、22.5和30mg/kg，給藥後加以觀察21天。分別在第一天、第二天、第三天、第一週、第二週與第三週探討小白鼠之旋轉運動能力、多項自發性運動行為及生理功能的變化。小白鼠之旋轉運動能力是在旋轉跑步機 (Rotarod treadmill)中執行。多項自發性運動行為，則在自發性運動行為紀錄儀 (Digiscan system) 中進行。PMA只在30mg/kg計量下，才具統計意義的降低小白鼠之重複行為 (重復性行為時間、重複性行為數目、重複性行為次數) 。在給予PMA22.5mg/kg與30mg/kg後的第一、二、三天，二種計量皆顯著的降低小白鼠之體重和造成死亡。既然PMA可降低小白鼠腦邊緣區之DOPAC濃度和股直肌之肌電位，所以PMA降低小白鼠之重複行為和旋轉運動能力的毒性，包括作用到中樞神經以及末稍神經，而且我們推測PMA的傷害神經區域，至少包刮了中腦－腦邊緣系多巴胺系路徑以及膽鹼激素系路徑。
Phenylmercury acetate (PMA) was given by single subcutaneous (SC) administration in adult male lice at doses of 7.5, 15, 22.5, and 30 (mg/kg), respectively. The mice were then observed for 21 days. Rotarod performance and multivariate locomotor behaviors of mice were tested on the rotarod treadmill and with the Digiscan system at 1st day, 2nd day, 3rd day, 1st week, 2nd week, and 3rd week, respectively. PMA at dose of 30 mg/kg significantly decreased stereotypy behaviors (stereotypy time, stereotypy counts, and stereotypy number). PMA at doses of 22.5 mg/kg and 30 mg/kg markedly decreased rotarod performance and urination on the 1st, 2nd, 3rd day after administration of PMA. In addition, PMA was found to decrease body weight and cause death in mice in a dose-dependent manner. Since PMA significantly decreased the concentration of DOPAC in the limbic area and attenuated the potential of integral electromyograph (IEMG) in rectus femoris muscle in mice, the results appear to show that PMA decreases the number of stereotypy behaviors and that rotarod performance maybe include both central and peripheral sites. Therefore, we tentatively assumed that neurotoxic sites.of PMA include the mesolimbic dopaminergic pathway and the cholinergic pathways at least.