探討Lactobacillus rhamnosus GG 對於DerP 致敏鼠的療效評估

文明進展促使生活更加便利，但卻也為環境帶來更多負擔，導致氣喘的發生率逐年增加。針對氣喘的新藥不斷開發，卻無法達到根治的效果。益生菌（Probiotics）為對宿主有益的微生物，具有調節腸道內微生物平衡，調控免疫微環境之能力，並進而緩解過敏發炎情形。本研究使用BALB/c母鼠以塵蟎蛋白 (Der P)誘發小鼠產生過敏反應。於實驗第1~3天對母鼠進行Der P腹腔注射，誘發致敏反應，而於第14、17、21、24及27天則進行Der P鼻腔吸入。本研究將致敏小鼠隨機分為2組，一組於實驗第1~14天餵食LGG(Lactobacillus rhamnosus GG)，探究LGG對於氣喘預防的效果，另一組則於實驗第14~27天餵食LGG，探究LGG對於氣喘治療效果。實驗對照組則是以餵食生理食鹽水進行。實驗結果顯示，致敏小鼠的呼吸道發炎反應現象會經由前後給予LGG而受到抑制，而其他相關生化數值，如IgE、Th2分泌細胞激素也獲得有效抑制，而IFN-γ與TGF-β則有顯著增加。藉由本研究結果可發現口服LGG對於Der P致敏的呼吸道炎症有預防及治療的效果，故對於過敏性疾病/氣喘疾病，在未來臨床上應可做為輔助治療或預防的選擇。

Asthma is one of the most common chronic inflammatory diseases. People with asthma have sensitive airways which react to triggers causing the mucosa swelling and airways narrowing. The incidence of asthma is increasing every year. There is more and more research for asthma therapy and probiotics is one of them. Probiotics are microorganism which can provide health benefit to host, regulate microbial balance in the intestine, control immune microenvironment and alleviate the condition of allergy and inflammation. In this study, we used Dermatophagoides pteronyssinus (Der P) to induce female BALB/c mice allergic reaction. The mice received intraperitoneal Der P sensitization on day 1 to day 3 and intranasal Der P sensitization on day 14, 17, 21, 24, and 27. This animal asthma model had been established. Besides, the Der P -sensitized mice was randomly assigned to two groups. One was fed LGG (Lactobacillus rhamnosus GG) on day 1 to 14 to test the prophylactic effect to asthma; the other one was fed on day 14 to 27, to test the treatment effect to asthma. The normal control group was the non-sensitized mice who received normal saline rather than Der P. According to the study, LGG treatment group, no matter before or after Der P sensitizing, can suppress the airway inflammatory reaction, reduce IgE and Th2 cytokines and raise the IFN-γ and TGF-β. In conclusion, oral LGG can prevent and treat Der P -sensitized airway inflammatory reaction. Therefore, oral LGG may has a role in allergic airway disease prevention and treatment.