大蒜之五種水溶性含硫化合物在動物試驗中抗氧化活性之研究

中文摘要： 以動物試驗來探討cysteine及大蒜中五種水溶性含硫化合物(N-acetyl cysteine、 S-allyl cysteine、S-ethyl cysteine、S-methyl cysteine 、S-propyl cysteine)，在小白鼠肝、腎及血漿中，對麩胱苷肽 (glutathione)、三酸甘油酯 (triglyceride)、總膽固醇 (total cholesterol) 及纖維網狀蛋白 (fibronectin)濃度、麩胱苷肽過氧化酶 (glutathione peroxidase)及過氧化氫酶 (catalase)活性的影響。於老鼠飲水中添加cystiene或五種含硫化合物(1g/L) 四週後，cysteine組及五種含硫化合物組，在肝、腎中的麩胱苷肽濃度，皆顯著高於控制組 (p<0.05)。與控制組比較，cysteie會降低肝中麩胱苷肽過氧化酶及過氧化氫酶的活性 (p<0.05)、增加肝、腎、血漿中，以Fe2+ 或glucose誘發的脂質過氧化 (p<0.05)， 和增加血漿中三酸甘油酯及總膽固醇的濃度(p<0.05)。與控制組或cysteine組比較，五種含硫化合物會增加麩胱苷肽過氧化酶及過氧化氫酶在肝、腎及血漿中的活性 (p<0.05)、降低肝、腎、血漿中，以Fe2+ 或glucose誘發脂質過氧化 (p<0.05)，和降低血漿中三酸甘油酯、總膽固醇、纖維網狀蛋白的濃度(p<0.05)。本研究顯示，大蒜中五種含硫化合物具有顯著的抗氧化性，對預防或治療氧化相關疾病應該有所幫助。 關鍵字：含硫化合物、過氧化氫酶、三酸甘油酯

Abstract: Balb/cA mice were used to study the in vivo effect of N-acetyl cysteine, S-allyl cysteine, S-ethyl cysteine,S-methyl cysteine and S-propyl cysteine,all derived from garlic,on glutathione (GSH) concentration and catalase and glutathione peroxidase (GPx) activities in plasma, kidney and liver. Cysteine was used for comparison. The effects of these compounds on the level of fibronectin, triglyceride (TG), and cholesterol were also evaluated. Cystiene or cysteine-containing compounds were added to drinking water at 1 g/L. After 4 wk of treatment, GSH levels in kidney and liver were greater (p<0.05) than in controls. Cysteine decreased catalase and GPx activities in liver, and enhanced both Fe2+- and glucose-induced lipid oxidation in plasma, kidney, and liver compared with the control group (p<0.05). However, the administration of the five cysteine-containing compounds enhanced catalase and GPx activities in kidney and liver, and reduced Fe2+- and glucose-induced lipid oxidation in plasma, kidney, and liver compared with the control and cysteine-treated groups (p<0.05). Intake of the five cysteine-containging reduced fibronectin, TG and cholesterol concentration in plasma and liver compared with the control and cysteine-treated groups (p<0.05).The five cysteine-containing compounds derived from garlic had marked effects on antioxidant enzymes in mice. Furthermore, these compounds reduced fibronectin, TG and cholesterol concentrations in plasma. These data indicate that these compounds have protective effects for cardiovascular disease prevention. Keywords: cysteine-containing compounds, catalase, triglyceride