黃岑對非小細胞肺癌影響之探討

肺癌是全球目前死亡率最高的惡性腫瘤，其中約八至九成是屬於非小細胞肺癌，現今多以手術合併化學或放射療法為主。為了減少病患接受治療所承受的負擔，天然化合物成為目前癌症治療發展的主流。根據古文典籍記載，黃岑作為中藥已有數千年的歷史，至今仍被應用於臨床上常見的病症。除了傳統上已知具有抗菌、抗感染及抗發炎等療效，最新的研究證據表明黃岑具有抗腫瘤的功效。有鑑於目前黃岑對於人類非小細胞肺癌—特別是惡性肺腺癌以及惡性大細胞肺癌之影響，缺乏相關文獻，因此本研究探討黃岑萃取物對於人類非小細胞肺癌的影響。本研究以不同濃度之黃岑萃取物處理肺癌株H460和H1355 細胞，結果發現萃取物有效抑制細胞增生、細胞爬行的能力。透過流式細胞儀分析，也發現細胞周期中sub G1之比例顯著增加；由西方墨點法分析，發現LC3-II與ATG3等細胞自噬相關蛋白表現顯著上升；利用免疫螢光染色分析，發現胞內產生自噬小體。綜合以上結果，黃岑萃取物可誘導非小細胞肺癌H460和H1355 細胞株進行細胞自噬，進而抑制細胞生長。

Lung cancer has been proven to bear the highest mortality rate among malignant tumors, with approximately 90% of the cases categorized as and non-small cell lung cancer (NSCLC). In order to reduce agony caused by conventional treatment — surgery combined with chemical or radiation therapy, the study of traditional Chinese herbs (TCM) towards cancer has recently gained its significance. According to ancient records, Scutellaria baicalensis has been applied as a remedy towards common disease for thousands of years. Moreover, latest proofs indicate that S. baicalensis is effective in inhibiting tumoral growth.However, the effect of S. baicalensis on human NSCLC, especially lung adenocarcinoma and large cell lung cancer, has not yet been inquired in previous studies. Hence, the effect and mechanism of S. baicalensis extract on human NSCLC were investigated in this research. We found that of NSCLC cells treated with S. baicalensis extract demonstrated significantl reduction in cell viability and cell migration. Through flow cytometry analysis, S. baicalensis extract has resulted in the increase of sub-G1 ratio. Western blotting reveals the increase in expression levels of LC3-II and ATG3 protein which are associated with autophagy pathways. Besides, cancer cells treated with S. baicalensis extract produuces intracellular autophagosome, successfully captured by Immuno-fluorescence analysis. Combining all the mentioned results, S. baicalensis extract triggers the autophagy process within H46 and H1355 tumoral cell lines, therefore successfully hindering cancer development.