巴西蘑菇與台產洋菇之抗氧化作用比較研究

本研究是利用巴西(Bralei)及台灣產的兩種蘑菇，各以10％和20％不同濃度的處理之下，與AIN-76飼料一起混合飼育小白鼠，目的在比較對於實驗動物抗氧化能力是否有不同的影響。 ICR品系成熟齡的小白鼠經由上述方式飼育經過一個月，其體重均有隨著週齡的增加各組亦呈增長的情形，由此顯示蘑菇的飼育並不影響小白鼠的生長。在我們的實驗期間，飼料不採自由取食的方式，而是以每日每隻mice固定給予5克攝食量的方式給食，因此其體重呈現穩定的增加，且實驗動物不易有過胖、脂肪肝的問題，而影響實驗結果的呈現。血漿中脂質過氧化產物MDA的濃度，藉以評估蘑菇的攝取對實驗動物脂質過氧化程度的影響。發現服用巴西蘑菇的組別MDA值皆有明顯下降的現象，尤以服用六週後最為明顯。在內毒素所誘發的氧化壓力(oxidative stress)之下，有效的降低自由基對身體造成的傷害，具有抗氧化的功效。血漿中具有抗氧化及分解作用之Glutathione及Catalase濃度也均有上昇之趨勢。所以我們假設，蘑菇的攝取其可降低脂質過氧化物的機轉，是透過了幫助小白鼠提高其體內Catalase抗氧化酵素的活性來達到，並藉此來對抗細菌內毒素所誘發的氧化壓力。同時也顯示了巴西蘑菇抗氧化作用方面優於台灣產蘑菇。

Agaricus blazei (from Brazil) and Agaricus bisporus (from Taiwan) at10% and 20%were used to mix with AIN-76 feeds in ICR mice to study their antioxidant protection.In each group, 5-g-feed was given to each mouse every day. After 1month feeding, mice bodyweight increase were not different among groups. This result showed that Agaricus blazei and Agaricus bisporus did not affect growth of mice and their bodyweight.Plasma oxidation level, determined as malondialdehyde(MDA), was measured to evaluate their antioxidant effect. Mice consumed Agaricus blazei showed significantly lower MDA level, especially at 6 weeks. Under endotoxin-induced oxidative stress, mice consumed Agaricus blazei showed significantly greater protection against free radical-induced oxidation damage. Plasma glutathione level and catalase activity in mice consumed Agaricus blazei were significantly elevated.Thus, we concluded the intake of Agaricus blazei and Agaricus bisporus makedly improved antioxidant protection via increasing the activity of antioxidant enzymes such as catalase against endotoxin-induced oxidative damage. This present study found that the antioxidant protection from Agaricus blazei was greather than Agaricus bisporus.Keywords: Agaricus blazei , Agaricus bisporus, glutathione, catalse, malondialdehyde