甲基安非它命對大鼠坐骨神經許旺氏RSC96細胞增殖的影響

甲基安非他命會導致人體永久異常的腦結構改變和加速老化有關，並未了解。許旺氏細胞 (Schwann cells; RSC96) 在周邊神經系統中包圍神經軸突形成髓鞘，與動作電位傳導有關，並參與軸突生長，變性等重要神經生理功能，保護神經元本體及軸突免於各式傷害。而如果許旺氏細胞受到病理性的傷害，則會形成神經的去髓鞘，正常的神經生理功能維持也會受影響以及軸突傳導的減慢。許多臨床疾病造成周邊神經病變都與許旺氏細胞的病理變化有關，例如外傷性脊髓損傷，糖尿病周邊神經病變，化學治療藥物導致的神經病變，以及慢性重金屬中毒導致的周邊神經病變，故許旺氏細胞的病理現象及凋亡在周邊神經病變中佔有重要地位。本實驗目的主要探討甲基安非他命抑制許旺氏細胞生長的機制，以確認甲基安非他命與加速腦細胞老化的關係。我們用不同濃度的甲基安非他命 ( 0, 0.7, 1.4, 2.1 and 2.8 mM) 處理RSC96細胞，48小時之後我們發現細胞存活率下降，分別為100%, 83%, 81%, 65%和61%。而利用flow cytometry測定發現細胞生長停在G0G1期，所以甲基安非他命對RSC96 細胞所造成的影響可能與細胞衰老 (cell senescence) 有關。

Methamphetamine can cause structural changes in the brain related to the human body and accelerate the aging of permanent exception, did not understand. Schwann cells (Schwann cells; RSC96) in the peripheral nervous system axons surrounded by myelin formation, and action potential conduction-related, and participate in neurite outgrowth, nerve degeneration and other important physiological functions, protect neurons body and axon Free in all kinds of damage. And if Schwann cells are pathological damage to nerve myelin is formed, to maintain the normal physiological function of the nerve is also affected and slowed axonal conduction. Many clinical disease caused by peripheral neuropathy and pathological changes are related to Schwann cells, such as traumatic spinal cord injury, diabetic peripheral neuropathy, chemotherapeutic drug-induced neuropathy, and chronic heavy metal poisoning caused by peripheral neuropathy, it Schwann pathological phenomena and apoptosis''s cells plays an important role in the peripheral neuropathy. The purpose of the experiment focuses on methamphetamine Schwann cell growth suppression mechanism to confirm methamphetamine and accelerated aging of the relationship between brain cells. We deal RSC96 cells with different concentrations of methamphetamine (0, 0.7, 1.4, 2.1 and 2.8 mM), after 48 hours we found decreased cell viability, were 100%, 83%, 81%, 65% and 61%.The use of flow cytometry cell growth assay was found parked in the G0G1 phase, so the influence of methamphetamine caused RSC96 cells may be related to cell senescence (cell senescence) concerned.