綠薄荷抑制人類骨肉瘤細胞增生及移動能力之機制探討

骨癌是罕見的惡性腫瘤，為一種侵犯骨頭的癌症，分為原發性骨癌及轉移性骨癌，在原發性骨癌中最常見之病理診斷為惡性骨肉瘤，好發於青少年與年輕的成人，治療方式通常以手術切除合併化學治療以達到良好的預後，但是骨肉瘤生長速度快且容易發生遠端轉移，也容易產生治療抗性，因此急需尋找新的藥物來抑制骨肉瘤的生長及轉移能力，而薄荷被廣泛的添加在食品及飲品中增加風味，也會提取其中的成分來製造成藥品、精油，以達到提神、舒緩的功效，在傳統醫學中更是被拿來添加在各種藥方中治療疾病，因此我們想使用綠薄荷50%酒精萃取物來探討綠薄荷是否具有抑制骨肉瘤生長及移動的能力。在本研究中，我們首先使用MTT assay來確定綠薄荷不會有毒殺人類骨肉瘤細胞143 B與U-2 OS的作用，接著再使用zymography來看綠薄荷是否會抑制MMP-2與u-PA的酵素分泌量，再以傷口癒合實驗以及Boyden chamber來探討綠薄荷是否會抑制骨肉瘤細胞移動與侵襲的能力，實驗的結果顯示綠薄荷可以抑制骨肉瘤細胞移動的能力，最後以西方墨點法來探討綠薄荷抑制人類骨肉瘤細胞存活、生長以及移動的途徑可能經由FAK/paxillin信號途徑。

Bone cancer is a rare malignant tumor, which is a kind of cancer invading bone. It can be divided into primary bone cancer and metastatic bone cancer. The most common type of primary bone cancer is osteosarcoma, which usually occurs in children and adolescents. The treatment is usually surgical resection combined with chemotherapy to achieve better prognosis. However, osteosarcoma grows fast and often metastasis, and it is also with treatment resistance. Therefore, it is acquired to find new drugs to inhibit the growth and metastasis ability of osteosarcoma. Mentha has long been used in various forms and is widely added in food and drink to add flavor, also originally used as a medicinal herb to treat stomachache and chest pains in traditional medicine. Thus, we wanted to explore whether Mentha spacita ethanol extract (MSE) has the ability to inhibit the growth and motility of osteosarcomas using a 50% extract of Mentha spacita.In this study, we first used the MTT assay to determine whether MSE would not toxicly kill human osteosarcoma 143 B and U-2 OS cell, and then use zymography to demonstrate if MSE inhibits enzyme secretion, and then used wound healing and Boyden chamber assay to explore whether MSE inhibits the migration and invasion of osteosarcoma cells. Finally, western blot was used to investigate the possible pathway of MSE inhibiting the survival, proliferation and migration of human osteosarcoma cell.Our study indicates that as the concentration of MSE may increase the ability to inhibit the motility of osteosarcoma cell. Finally, the western blot was used to explore the possible ways that can inhibit the survival, growth and migration of human osteosarcoma may be FAK/paxillin signal pathway.