維他命E和維他命C補充對慢性阻塞性肺疾病患的影響

本實驗的目的，是為了了解維他命E及維他命C的補充，是否能改善慢性阻塞性肺疾(Chronic obstructive pulmonary disease)病人全血白血球的DNA受損情形。三十五位病況穩定的COPD病患以隨機對照的方式(Randomized and Placebo controlled study)進行研究。受試者隨機分配在安慰組(n＝ 8)、維他命E 組400毫克/天(E 400, n=9)、維他命 E 組200毫克/天(E 200, n=9)、維他命 C 組250 毫克/天(C250, n=9)共進行十二週的試驗。實驗結果顯示，補充維他命E或維他命C對於內源性DNA沒有顯著的影響；然而經過為期十二週維他命補充劑的補充，在E400、E200及C250各組，過氧化氫所誘發的DNA傷害抑制率分別為45 ％、59 ％以及52 ％ (p＜0.05)；另外，不論是TBARS(thiobarbituric acid reactive substances)或是肺量計參數值 (spirometric parameters)在十二週維他命補充劑使用後，各組間並沒有顯著的差異。雖然未來仍需要更多的研究來驗證維他命E或C的補充是否能有效的延緩COPD病患的肺功能衰退，不過本研究顯示，維他命E或C的補充可以改善全血白血球DNA對於氧化壓力的耐受性。

The purpose of this study was to determine whether vitamin E or vitamin C supplementation alters the DNA damage of whole blood white blood cells (WBC) in patients with chronic obstructive pulmonary disease (COPD). Thirty-five patients with stable COPD were recruited in this randomized and placebo-controlled study. Patients were randomly assigned to placebo (n = 8), 400 mg/d vitamin E (E400, n = 9), 200 mg/d vitamin E (E200, n = 9) or 250 mg/d vitamin C (C250, n = 9) for 12 weeks. The results showed that vitamin E or C supplementation did not significantly change the mean level of endogenous DNA breakages. Whereas, after 12 weeks of vitamin supplementation, the H2O2-induced DNA breakages were significantly suppressed by 45%, 59% and 52%, respectively, in E400, E250 and C250 groups (p<0.05). In addition, neither the level of TBARS nor spirometric parameters were significantly changed after 12 weeks of supplementation. In conclusion, vitamin E or C supplementation for 12 weeks may improve the resistance of DNA in whole blood WBC against oxidative challenge, although more research is needed to demonstrate the beneficial effect on slowing the decline of lung function in patients with COPD.