洗滌後精液的冷凍對精子解凍後產生負面影響

評估精液冷凍前洗滌（密度梯度離心法）與冷凍後洗滌之比較，在解凍過程後，何者有較佳的精子活動性、精子活性和凋亡精子的百分比。將50名男性的精液樣本各取1 毫升，其中一份在冷凍保存前將精液進行密度梯度離心法洗滌，一份在冷凍保存後進行精液密度梯度離心法洗滌。測定各組精子數量、活動性、活力和凋亡指數。結果發現，與原精液組相比，冷凍前洗滌組和冷凍後洗滌組在解凍後總精子數和總活動精子數均減少(P<0.001)。此外，冷凍後洗滌組精子去氧核醣核酸碎片指數明顯比凍前洗滌組低（P=0.0013），而解凍後的活動精子總量，與具有向前活動的活精子總量，兩組間則無統計上的差異。結論，精液冷凍保存雖會對精子參數產生不利影響。但不論是活動精子的總量與向前性活動精蟲，冷凍前洗滌與冷凍後洗滌結果一致。因此冷凍前洗滌或冷凍後洗滌皆可選擇用來做為臨床之使用。

To evaluate whether semen preparation before freezing (gradient density centrifugation) and after cryopreservation have better sperm motility, sperm motility, and percentage of non-apoptotic sperm after freezing and thawing.The semen samples of 50 men were divided into two equal parts, one of which was washed with gradient density centrifugation before cryopreservation, and the other was washed after cryopreservation. The sperm count, motility, vitality and apoptosis index of each group were measured.Compared with the original semen group, the total sperm count and the total motile sperm count of the pre-prepared group and the non-prepared group were reduced after thawing (P<0.001). In addition, after cryopreservation, the percentage of apoptotic sperm in the pre-prepared group was higher than that in the non-prepared group (P=0.0013), while the total amount of viable sperm with progressive movement was not statistically different between the two groups.Therefore it was concluded that sperm cryopreservation affects adversely semen parameters. After cryopreservation, sperm preparation after thawing resulted in the same sperm quality but the apoptotic sperm was fewer than sperm pre-prepared before cryopreservation. Therefore, wash before or after freezing can be selected for clinical use.