以動物模式驗證減藍光鍍膜鏡片用於減緩LED照明所造成視覺生理功能改變之分析

視覺疾病的威脅、視覺生理失調與眼睛疲勞症狀，將會成為一個當代普及的問題。而來自智慧型手機、平板電腦、家用電腦與LED照明之高能量的藍光被認為是引起這些視覺問題發展的因素之一。短波長藍紫光比其他波段的可見光，更容易傷害視覺系統。減藍光鏡片常使用於管理藍光量避免其所造成的視覺問題，然而，此用途之訴求在生物體之視覺生理系統的驗證研究並不完整。本研究目的在動物模式探討減藍光鏡片對於預防LED照明 (400-1000lux) 所引起的視覺生理功能下降的效果。ICR小鼠隨機分為4組：(1)空白對照組 (Blank control) ; (2) LED照射組 (LED組) ; (3) LED照射且使用無減藍光鏡片組 (LED + Un-BLC組) ; (4) LED照射且使用減藍光鏡片組 (LED + BLC組)。在此實驗模式下，本實驗分析策略採取視覺生理功能評估與視網膜組織分析方法進行驗證減藍光鏡片的預防效果。完成LED光照刺激後，與LED + BLC組小鼠相比，結果發現LED組與LED + Un-BLC組組小鼠的視力值明顯降低、延遲瞳孔對光反應的時間並且損失了感光細胞在視網膜組織結構上的完整性。雖然與Blank control組小鼠相比，發現 LED + BLC組的小鼠視力仍然有輕微下降的問題，但其視力值維持的程度比LED組與LED + Un-BLC組組來的佳，且有高度的顯著性。研究最後指出，小鼠視覺生理功能的表現與感光細胞損傷程度有高度的相關性。本研究證明減藍光鏡片在生物體之視覺生理系統，有助於視覺的健康。

Vision-threatening diseases, physiologic maladjustment of vision, and eye strain symptoms will become common problem. One cause of these problems is believed to be the high energy blue light emitted from the display on is considered as devices, such as smart phones, tablet devices, computers and LED lighting. Blue light with short wavelength is more harmful to visual system than the other wavelengths of visual light. The blue light cutting spectacle lens are usually used against the blue light related vision problems, but it’s efficacy in the bio-physiological visual system is not well studied. This study aims to the preventive effects of spectacle lens coating for blue light cutting and against the LED lighting (400-1000lux) induced declining of visual physiological function in vivo. ICR mice were randomly divided into 4 groups: (1) normal blank control (Blank control group), (2) LED lighting exposure (LED group), (3) LED lighting exposure group under protection with Un-Blue-Light Cutting spectacle lens (LED + Un-BLC group), (4) LED lighting exposure group under protection with Blue-Light Cutting spectacle lens (LED + BLC group). The visual physiological function assessment and retinal histological analysis were performed to evaluate the preventive effects of blue light cutting spectacle lens in the mouse model. After LED lighting exposure, the mouse showed significant decline of visual acuity, delay of pupil reflex time, and loss of cell integrity of photoreceptor in the retinal tissue in the LED group and LED + Un-BLC group, as compare to LED + BLC group. Although, slight degree of visual acuity was also occurred in the LED + BLC group as compare to blank control group, visual acuity of the LED + BLC group was more still better than LED group and LED + Un-BLC group. In addition, mush there is higher correlation between the decline of visual acuity and the degeneration of retinal photoreceptor. Taken together, this study demonstrated the spectacle lens with cutting blue light properties are helpful for visual health in the bio-physiological system in vivo.