桑葉萃取物及新綠原酸抑制糖毒性誘導之糖尿性心肌病作用

糖尿病是現代很常見的一種慢性病，會造成許多的併發症，例如腎臟病變、視網膜病變、心血管病變以及神經病變等等，本篇研究會以心臟病變方面來探討。由糖尿病引起的糖尿病性心肌病Diabetic cardiomyopathy（DCM）是由於心臟長期暴露在高血糖環境下造成的，其造成的異常主要有：增加細胞凋亡、發炎反應、氧化壓力和細胞間質纖維化等等，最終造成心衰竭導致死亡。桑葉萃取物（MLE）有許多研究證實可以改善動脈粥狀硬化，降低心血管疾病，但是對於減緩糖尿病的心肌損傷的作用還尚未有研究做深入探討。而在桑葉萃取物（MLE）中含量最多的多酚類是新綠原酸（nCGA），因此本實驗將藉由動物實驗以及細胞實驗兩方面，來研究MLE以及nCGA是否可以減少由糖尿病所引起的心肌損傷。動物實驗方面利用MLE與nCGA餵食db/db第二型糖尿病小鼠，實驗期間每週紀錄實驗動物之體重變化以及血糖變化。細胞實驗則是使用糖化終產物（AGEs）來進行誘導，並分別加入MLE與nCGA來觀察是否可以改善細胞凋亡以及氧化壓力的作用。結果顯示MLE與nCGA均可以藉由減少氧化壓力以及增加抗氧化能力達到抑制細胞凋亡的發生。

Diabetes is one of the most common chronic disease in modern society, which can cause many other complications, such as kidney disease, retinopathy, cardiovascular disease and neuropathy, etc. This study is going to discuss the heart disease. Diabetic cardiomyopathy (DCM) caused by long-term exposure of the heart to hyperglycemia, commonly leads to increased apoptosis, inflammatory response, oxidative stress and interstitial fibrosis, and so on. The abnormalities will eventually cause heart failure and death. Many studies had confirmed that Mulberry leaf extract (MLE) can improve atherosclerosis and reduce cardiovascular disease, but neither of them studied the effect of reducing myocardial damage in diabetes in depth. The most abundant polyphenol in mulberry leaf extract (MLE) is neochlorogenic acid (nCGA). Therefore, this experiment will use both animal and cell experiments to study whether MLE and nCGA can reduce diabetes-induced diabetes. In animal experiments, MLE and nCGA were used to feed db/db type 2 diabetic mice, and recorded the changes in body weight and blood glucose of the experimental animals weekly. In cell experiments, end-products of glycation (AGEs) were used for induction, and add MLE and nCGA separately to see if it could reduce apoptosis and oxidative stress. The results showed that both MLE and nCGA could inhibit the occurrence of apoptosis by reducing oxidative stress and increasing antioxidant capacity.