香蕉皮正己烷萃取物之類胡蘿蔔素組成及其生物活性

香蕉為是全世界消費量最大的水果。香蕉有促進消化、抗憂鬱、抗氧化、抗癌等功能，香蕉經成熟後果皮的顏色會轉黃，可能含有多量的類胡蘿蔔素。因此本研究除測定香蕉皮中類胡蘿蔔素的種類及含量，並測定其生物活性。本研究取用台灣產北蕉的果皮及果肉，分別取低極性正己烷(n-hexane)萃取物、中極性乙酸乙酯(ethylacetate)(EA) 萃取物、高極性水(water) 萃取物進行生物活性之探討。結果顯示，香蕉皮的抗氧化性明顯優於香蕉肉，而香蕉皮低級性萃取物具有最佳之抗氧化性與清除自由基的能力。 香蕉皮低極性萃取物中含有α-carotene、ß-carotene、lutein等類胡蘿蔔素，其中又以lutein的含量最多(0.52mg/g dry wt.)，以藍光(430±30nm)照射 ARPE-19細胞，造成細胞損傷之模式顯示，香蕉皮之低極性萃取物具有明顯保護細胞並使細胞增生的作用。 此外，香蕉皮低極性萃取物能夠有效抑制KB及ARPE-19細胞cyclooxygenase 2之表現，並且可促進KB細胞發生凋亡的現象。

Banana is greatly consumed in the world. Banana shows digestive promotion, anti-depression, anti- oxidation, and anticancer, Mature banana peel contains rich carotenoids, This study was to determine the carotenoids of the banana peel, and also its biological activities.Taiwan Pei Chial was used in this study Low-polar (n-hexane),semi-polar (ethylacetate) and high-polar (water) extracts were obtained for discussing the biological activities. Results showed that banana peel had better antioxidation than that of pulp. Low-polar (n-hexane) extract of banana peel showed perfect antioxidation and scavenging efficiency for free radicals. Low-polar extract of banana peel included α-carotene ß-carotene and lutein, and the levels of leutin were very high. In blue light exposure MTT assay test, blue light (430nm±30) could destroy ARPE-19 cell (human retinal pigment epithelial cell line). Low-polar extract of banana peel extract could restore the damage of ARPE-19 cell. In addition, Low-polar extract of banana peel inhibited the expression of COX-2 of KB cell and ARPE-19 cell. Low-polar extract of banana peel also induced KB cell apoptosis.