Inhibition of neurite outgrowth and promotion of neuronal degeneration by the atropine in Neuro2a cells

Myopia, a very common human eye disorder, affects up to 80% of young adults in some East Asian countries, including in Taiwan. Higher degree of myopia is associated with a number of visual terrorized complications, including retinal detachment, macular degeneration, cataract and glaucoma. The risk of these complications rises with increasing severity of myopia. Contrasting other blinding disorders such as aged-related macular degeneration, all these myopia-related complications tend to arise mainly in young adult. Atropine, a pan muscarinic cholinergic receptor antagonist, has been used for treated myopia since long time ago. However, the influence of innervation in the eyes was still not known. To address this question, the effect of atropine on nerve innervation and cell character, the pan muscarinic cholinergic receptor antagonist treated cultured cell were examined. The neuronal neurite lengths were assay with microscopy. To study the relationship between neuronal death and atropine-treated, statistical analysis was examined. Blocked the neuronal transmission caused the cells neurite lengths decreased compared with control. It also detected that the increase in the number of nuclear condensation cells after treated atropine with microscopy. In other words, neuroblastoma cells after atropine-treated, the cultured cell death ratio was increased compared with control. These results indicated that neuronal cells treated with atropine, a muscarinic receptor antagonist, reduced the neurite outgrowth and promoted neuronal degeneration.