探討荖葉對於熱氧化沙拉油/高膽固醇飲食下大鼠血脂、肝功能與主動脈的影響

熱氧化的油所產生的活性氧與一些疾病是有相關性的，包括高血壓、脂肪肝、心血管疾病等。而對於荖葉的研究當中，已經證實了具有抗氧化、護肝的效用。本研究的目的是探討荖葉對於使用油炸過的沙拉油餵食大鼠後對其血脂、肝功能以及心血管的影響。Sprague-Dawley 雄性大鼠隨機分為5組，分別餵食以下飼料，為期十週：正常飼料(Control)、高油脂沙拉油組(USO)、高油脂油炸沙拉油組(FSO)、高油脂油炸低劑量荖葉組(FSOL, FSO + Piper betle [50 mg/kg])、高油脂油炸高劑量荖葉組(FSOH, FSO + Piper betle [100 mg/kg])。十週後，犧牲大鼠，採集血液作血液生化值分析並將其肝臟、主動脈弓進行免疫組織染色法分析。實驗結果顯示，荖葉有降低血液中的LDL與膽固醇，減少肝臟中的三酸甘油脂與膽固醇的堆積以及體脂肪的重量現象。本次實驗雖然未能立即的觀察到荖葉對於血脂、肝功能以及心血管方面的效果，但是潛在的效果仍然是有的。所以本實驗可以作為未來研究方向的參考基礎。未來針對部分實驗細節作修正；例如：增加油炸油的油炸時間、油炸物的挑選、調整飼料比例以及增加實驗鼠數量。修正過後可望有更明確的結果出現。

Thermally oxidized oil generates reactive oxygen species that have been implicated in several pathological processes including hypertension, fatty liver, cardiovascular disease. For Piper betel L. (PBL) studies, has been confirmed to have antioxidant, hepatoprotective effect. Study of effects of PBL on serum lipids, liver function & aorta in rats fed with thermally oxidized soybean oil/ high-cholesterol diets. Male Sprague-Dawley rats were divided into four groups and were fed with the following diets, respectively, for 10 weeks: basal diet (control); Unused soybean oil diet (FSO); Soybean oil repeatedly used in fring diet (FSO); FSO diet + Piper betle [50 mg/kg] (FSOL); FSO diet + Piper betle [100 mg/kg] (FSOH). After 10 weeks, the rats were sacrificed, and the aortic arches were dissected for morphometric and immunohistochemical analyses. So from the experiment we suggest that PBL can reduce LDL and cholesterol in the blood, reduce the total cholesterol and triglyceride in the liver and the accumulation of body fat. Although this experiment failed to observed that an immediate effect on betel leaf for serum lipids, liver function, and cardiovascular, still have the potential. So this experiment can be used as a reference basis for futures studies. Future will make the revision in view of the part experimental detail. For example, increases the thermally oxidized soybean oil to fry in oil the time, to choose fry in oil the things, adjustment feed proportion, increase experiment rats quantity. The revision will hope to have the more explicit result.