利用乾式試片專一性偵測全血中的高密度脂蛋白膽固醇

高密度脂蛋白(HDL)在人體的循環系統中扮演著重要的角色，HDL藉由脫輔基蛋白A1(Apolipoprotein A1)和心臟、血管和細胞中的受體ATP-binding cassette transporter(ABCA1)結合，使細胞中的膽固醇流入HDL中並送回肝臟代謝，此途徑稱為reverse cholesterol transport”，也是HDL能改善心血管問題和預防疾病發生的原因，對於高血脂患者或是具有遺傳性高血脂病史的人是否須接受治療的一個重要參考依據。目前檢測高密度脂蛋白膽固醇大都採用生化分析儀，但其有著耗時、費用較高且需要前處理等問題。本文利用一個較具有專一性與HDL反應的界面活性劑(Surfactant)，溶解HDL particle進而使高密度脂蛋白膽固醇(HDL-c)流出，並利用膽固醇氧化酶(COD)、膽固醇酯水解酶(ChEs)和過氧化氫酶(POD)三種酵素與電化學反應系統，當全血與試片中所含酵素及電子傳導媒介接觸反應20秒後，通以電壓後即會進行氧化還原反應，所得到之氧化還原反應電流，再藉由累計電流值換算為高密度脂蛋白膽固醇濃度。本篇實驗使用電化學法，開發高密度膽固醇試片檢測平台，此檢測方式能偵測到全血中30~130 mg/dL的HDL 範圍，且具有使用簡單方便、結果快速、可隨時自我測量等諸多優點，相當便利。

High density lipoprotein (HDL) plays an essential role in human circulatory system and cholesterol transport. In the presence of apolipoprotein A1 (Apo A1), HDL binds to the specific receptor ATP-binding cassette transporter (ABCA1) and promotes the pouring of cholesterol in heart and arteries, the so-called “reverse cholesterol transport”. Therefore, HDL level is used as a predictor for risk factor of cardiovascular disease and an important indicator of hyperlipidemic patients for receiving medical treatments or not. Nowadays, biochemistry analyzer is the most common approach to analyze the levels of HDL-c. However, it is not practical due to the time-consuming, high-expense process, and complicated sample pretreatment. Here we used a compound, Emulgen B66, that can be selectively and specifically to dissolve HDL particle in a short period of time. The method was based on a homogeneous assay for selective determination of HDL-C to develop the redox electron on test strip by peroxidase/H2O2 reaction. A dual functional test strip for HDL detection is available. This method is handy, low-cost, easy to carry out and quick to know the results.