大蒜水溶性含硫化合物於STZ誘發之糖尿病小白鼠抗纖維化作用之影響

糖尿病已是台灣地區最重要的慢性病之一，糖尿病的各種慢性併發症是病患死亡的主因，尤其是心血管方面的疾病。因此，本研究目的在探討飲水添加大蒜水溶性含硫化合物對於被誘發糖尿病小鼠是否有抗纖維化之作用。使用Balb/cA雄鼠經由尾靜脈注射streptozotocin(STZ; 200mg/kg BW)誘發糖尿病，確認誘發糖尿病成功後，將大蒜水溶性含硫化合物加入飲水中，分別為(1)非DM組(2)DM-Water (3)DM-Cystein (4)DM-NAC (5)DM-SEC (6)DM-SMC (7)DM-SPC，期間採自由飲水的方式。結果顯示，大蒜水溶性含硫化合物會使糖尿病小白鼠血漿中的血糖及血脂濃度與存在細胞間質的纖維網狀蛋白(fibronectin)有顯著性降低。飲用大蒜水溶性含硫化合物組別小鼠的纖維蛋白溶解系統（fibrinolytic system）中之t-PA（tissue-type plasminogen activator）有顯著性的上升，且MMP2與Plasminogen也都有降低之現象。發炎反應中SEC及NAC組小白鼠之IL-6降低較為顯著。由於糖尿病患長期處於高血糖會使其fibronectin上升及纖維蛋白溶解系統中的活化者下降，進而提高血管病變的機率；而由上述結果顯示出，當飲水中添加大蒜水溶性含硫化合物，可降低引發血管病變之血糖及血脂濃度與纖維網狀蛋白，並增加t-PA濃度，進一步預期可減緩血管方面的病變。

Diabetes mellitus is one of the most serious chronic diseases and its serial complications always cause death, especially those what cardiovascular system diseases. Therefore, we would like to confer the effects of cysteine-containing compound derived from garlic added in water against fibrosis development in STZ-induced Balb/cA mice. We tried to induce diabetes mellitus by injected streptozotocin(STZ) to Balb/cA male mice from their tail’s vein. Confirming induction succeeded, we added cysteine-containing compound derived from garlic into drinking water and fed the mice. This experiment was focus on 7 mice groups , which were（1）non-DM（2）DM-water（3）DM-cysteine（4）DM-NAC（5）DM-SEC（6）DM-SMC（7）DM-SPC. The results indicated cysteine-containing compound derived from garlic had decreased the blood sugar and the concentration of triglycerides in plasma and also the fibronectin content. In addition to that, the t-PA（tissue-type plasminnogen activator）content existed in fibrinolytic system of DM-cysteine mice had increased significantly, but MMP2 and plaminogen contents decreased. In inflammatory reaction, the DM-SEC and DM-NAC mice exhibited significant decrease in IL-6. There were researches indicated that DM patients maintained the high blood sugar for a long terms, their fibronectin content would increased and t-PA content would decreased. The situation would raise the probability of cardiovascular system diseases to occur. From the experiment data, we might expected that cysteine-containing compound derived from garlic should be used to prevent the happening of cardiovascular system diseases by its effects of decreasing the blood sugar、the concentration of triglycerides in plasma、fibronectin content and increasing the concentration of t-PA.