結冷膠/金聖草素微粒作為動脈栓塞劑之評估

腎臟癌一直是一種難以診斷和治療的惡性腫瘤，許多患者在診斷時就已有遠處轉移，在1970年代就有腎臟癌動脈栓塞治療的相關研究，現今被臨床應用在腎腫瘤、腎切除術前、緩解不可切除的腎癌、轉移性腎癌等。近年來也有許多利用栓塞劑作為藥物遞送系統的合併性療法，綜合了輸液和閉塞的優點，具有相當大的潛力，而結冷膠優於其他現有材料的特性，生物相容性、無毒、延展性等，可以作為栓塞劑和藥物遞送系統的載體。金聖草素是一種天然黃酮類，安全性高，能夠抑制癌症的生長，被也投入在乳腺癌、肺癌、多發性骨髓瘤等研究上。因此想探討以結冷膠為基材，包裹抑癌物質金聖草素，以乳化法製作出結冷膠/金聖草素微粒，作為可控釋放的栓塞劑。結冷膠/金聖草素微粒為分布均勻小孔洞的飽合球型，在產率與包覆率數值分別為60.4%和47.6%，血液相容性高。其釋放模式為Korsmeyer-peppas模式，因此應用在進行腎動脈栓塞時，血液可以透過結冷膠/金聖草素微粒填充微粒孔隙，使之膨脹並帶出金聖草素，達到靶細胞血液藥物濃度最大的治療效果。細胞存活試驗和細胞凋亡試驗顯示結冷膠/金聖草素微粒能有效抑制A498細胞的生長及誘導細胞凋亡。大鼠動物實驗可以看到結冷膠微粒血管栓塞在血管，並使腎臟面積縮小，不會導致過度發炎，對栓塞血管的動脈壁損傷小。故認為結冷膠/金聖草素微粒有潛力發展成動脈栓塞劑。

Renal cell carcinoma has always been a malignant tumor that is difficult to diagnose and treat. In the 1970s, there was renal artery embolization as therapy, and nowadays, researchers combine embolic agents and drug delivery systems because of the advantages of infusion and embolization. gellan gum is biocompatibility, non-toxicity, ductility, and it has been used as embolic agents and a carrier for drug delivery systems. Chrysoeriol is a natural flavonoid that it can against cancer. Therefore, in this study, gellan gum encapsulating chrysoeriol is formed to be microspheres by emulsification method as drug delivery system to embolic kidney. The gellan gum/ chrysoeriol microspheres are saturated spheres with small holes, with the yield rate and the coating rate are 60.4% and 47.6%, being high blood compatibility that its drug release kinetic mode is Korsmeyer-peppas mode. By cell survival test and cell apoptosis test, gellan gum/ chrysoeriol microspheres can effectively inhibit the growth of A498 cells to induce cell apoptosis. In the animal experiments, gellan gum microspheres embolize blood vessels and reduce the size of the kidneys without inflammation. In conclusion, gellan gum/ chrysoeriol microspheres have the potential to develop as embolization agents and a carrier for drug delivery systems.