3D列印含鍶矽酸鈣多孔支架於骨組織工程應用

鍶（Sr）被描述為對骨強度和結構具有有益的影響。然而口服藥物雷奈酸鍶會造成負面系統影響，導致臨床上的使用被受到限制。過去實驗室自行開發的矽酸鈣（CS）陶瓷，已證明該材料除了可以加速多種細胞的生長外，也能夠促進細胞進行硬組織分化。因此本研究將使用Sr取代CS中的Ca，已得到一新的陶瓷粉末。在本研究中，分別使用三種含鍶矽酸鈣陶瓷粉末（Sr0、Sr5、Sr10）與聚己內酯（PCL）作為3D列印的材料。將陶瓷粉末及PCL均勻加熱混合後，透過3D列印機製備成多孔支架已進行機械性質、降解性質以及細胞相關試驗。從結果可知陶瓷含量中的Sr含量增加會提升支架的機械強度，並且浸泡於仿生溶液七天後，也能於支架表面產生磷灰石沈澱。在細胞的觀測上，Sr於支架中的含量對於細胞生長並沒有顯著的提升，但是在鹼性磷酸梅及鈣沉積的分析中則能夠發現Sr10的組別較其他支架來得優異。由上述結果可得知，該陶瓷支架在未來於組織工程應用上是極具潛力的。

Strontium (Sr) is described as having a beneficial effect on bone strength and structure. However, oral administration of strontium ranelate can cause negative systemic effects, leading to limited clinical use. In the past, laboratory-developed calcium Silicate (CS) ceramics have proven that this material not only accelerates the growth of many kinds of cells but also promotes hard tissue differentiation of cells. Therefore, this study will use Sr to replace Ca in CS, and a new ceramic powder has been obtained. In the present study, three calcium tellurite containing ceramic powders (Sr0, Sr5, Sr10) and polycaprolactone (PCL) were used as 3D printed materials. After the ceramic powder and the PCL were uniformly heated and mixed, a porous scaffold was prepared through a 3D printer to perform mechanical properties, degradation properties, and cell-related tests. From the results, it can be seen that increasing the Sr content in the ceramic content will increase the mechanical strength of the stent, and after soaking in the bionic solution for seven days, it will also produce apatite precipitate on the surface of the stent. In cell observations, the content of Sr in the scaffold did not significantly increase cell growth, but in the analysis of alkaline phosphatase and calcium deposition, it was found that the Sr10 group was superior to other scaffolds. From the above results, it can be seen that the ceramic stent has great potential for bone tissue engineering applications in the future.