利用Phage Display 技術找出與 SARS-CoV-2 作用之胜肽

嚴重急性呼吸系統綜合症病毒 2（SARS-CoV-2）是一種新型冠狀病毒，經由血管緊張素轉換酶 2 (ACE2)與SARS-CoV-2的S蛋白結合感染人體，主要感染上呼吸道和其他組織/器官，包括咽、心、肝、腦、腎和胃腸道。Phage display是一個篩選胜肽或蛋白質的技術，利用胜肽設計夾帶在噬菌體蛋白質內，當與目標分子反應過後，經過淘選可以篩選出有結合的胜肽，這個與噬菌體結合的胜肽就是我們想要的目標胜肽。本實驗的研究目的是利用Phage display技術找到與CWVP相結合的胜肽，先將GST-C terminal CWVP和GST control組的蛋白質純化，純化過後與噬菌體展示技術的基因庫進行結合，然後將沒有結合的噬菌體洗掉，萃取出有結合在一起的噬菌體抽出DNA，再送去定序。送定序後我們得到了氨基酸序列，再去Translate Tools網站進行基因編碼。我們得到了23個結果，例如：CSSIPHARC、CHPRHLHLC、CLPPFSHMC…再對其進行分析，比較有沒有重複性較高的胜肽，如果得到重複性較高的胜肽，有可能根據結合的胜肽找到治療SARS-CoV-2的特效藥物或者製造出SARS-CoV-2的疫苗，也會成為我們未來實驗的新方向。

Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) is a new type of coronavirus that infects the human body through a combination of the angiotensin-converting enzyme 2 (ACE2) and the S protein of SARS-CoV-2, and mainly infects the upper respiratory tract and other tissues/organs, including pharynx, heart, liver, brain, kidney and gastrointestinal tract. Phage display is a technology for screening peptides or proteins, where the peptide is designed to be entrained in the phage protein. After reacting with the target molecule, the bound peptide can be screened after panning, with the peptide bound to the phage，resulting with our desired target peptide. The research purpose of this experiment is to use phage display technology to find peptides that bind to CWVP. First, purify the proteins in the GST-C terminal CWVP and GST control group. After purification, combine phage display technology’s gene library with the protein, then wash the unbound phages away, leaving the bound phages to be prepared for DNA extraction and sequencing are extracted to extract the DNA, and then sent for sequencing. After sequencing, we recieved the amino acid sequence, then used the Translate Tools website for gene coding analysis. We got 23 results, such as: CSSIPHARC, CHPRHLHLC, CLPPFSHMC... that we analyzed to observe whether there are peptides with higher repeatability. If peptides with higher repeatability are obtained, it can be found based on the combined peptides. Specific drugs for the treatment of SARS-CoV-2 or the creation of a vaccine for SARS-CoV-2 will become a new direction for our future experiments.