明膠-矽酸鈣高強度仿生移植材之性質

骨骼是世界上第二大最常被移植的組織，每年至少有400萬次手術使用骨移植物和骨替代材料，因此設計各種可降解高強度移植材是目前主要的研究之一。使用生物陶瓷與天然高分子的複合材料來模擬人體骨骼成分，因材料相關的感染導致臨床移植失效。為降低感染問題，本研究製備高強度明膠 - 矽酸鈣複合材料，並於外部披覆抗菌層，將幾丁聚醣與多酚類等抗菌劑塗覆在複合材料表面上。透過電子顯微鏡觀察支架表面形態，X光繞射儀與霍氏轉換紅外光譜分析結構與官能基團，利用抗壓強度、三點彎曲強度、徑向拉伸強度與衝擊強度分析機械性能，及評估體外浸泡行為。結果顯示含有10 wt％明膠的複合材料的抗壓強度值為140 MPa高於對照組的85 MPa，添加20 wt％明膠的材料則高達200 MPa，反而添加30 wt％明膠之抗壓強度降低至175 MPa，但仍然在皮質骨抗壓強度的範圍50-180 MPa內。衝擊強度與明膠含量成正比。三點彎曲強度和徑向拉伸強度均隨著明膠含量的增加而增加。在體外浸泡實驗中，在3個月後含有20 wt％明膠的複合材料仍然有124 MPa的抗壓強度，含30 wt%明膠的複合材料則是由於明膠含量過多，導致在兩周後支架膨脹瓦解。而在披覆層的評估上幾丁聚醣塗層浸泡一天內就完全降解，有添加多酚類能夠有效延長降解時間至7天後。綜合以上，多酚類披覆層的複合骨移植材有極大的潛力能夠應用於支架與骨折固定術上。

Skeleton is the second most commonly transplanted tissue in the world. Each year, there are at least 4 million surgical procedures use bone grafts and bone substitutes. Thus, designing various degradable high-strength graft materials is one of the major research fields. The usage of composites of bioceramics and natural polymers to simulate human bone components, often result in clinical graft failure due to material-related infections. In order to reduce the infection problem, this study targeted to develop a high-strength gelatin-calcium citrate composite material coated with antibacterial layer such as chitosan and polyphenols. Electron microscopy was used to observe the surface morphology. The structure and functional groups were analyzed by XRD diffractometer and FTIR spectroscopy. The mechanical properties were analyzed by compressive strength, three-point bending strength, radial tensile strength and impact strength. Vitro soaking behavior is also tested in the experiment. The results demonstrated that the compressive strength value of the composite containing 10 wt% gelatin was 140 MPa higher than that of the control group of 85 MPa. The material with 20 wt% gelatin was 200 MPa, but the compressive strength of the material was reduced to 175 MPa when adding up to 30 wt% gelatin. However, it is still within the range of 50-180 MPa of compressive strength of cortical bone. The impact of strength is proportional to the gelatin content. Both the three-point bending strength and the radial tensile strength increase as the gelatin content increases. In the in vitro immersion test, the composite with 20 wt% gelatin still had a compressive strength of 124 MPa after 3 months, and the composite with 30 wt% gelatin resulted in stent swelling and collapsed after two weeks because of excessive gelatin content. collapse. In the evaluation of the disclosure layer, the chitosan coating was completely degraded within one day, the addition of polyphenols could effectively prolong the degradation time to 7 days. In summary, composite bone grafts with polyphenolic coatings have great potential for stenting and fracture fixation.