護眼保健素材開發驗證：(1) 探討丹蔘酚酸B對於紫外線B誘導角膜損傷的保護作用。(2) 探討黃耆甲苷IV對於強光誘導視網膜退化的保護作用。

中文摘要 (一) 丹蔘 ( Salvia miltiorrhiza ) 為知名的傳統中草藥，具有活血化瘀、涼血消廱、養血安神等特性，已廣泛的使用於日常生活中。隨著現代藥理發展，發現其分離產物「丹蔘酚酸B (Salvianolic acid B, SaB)」，具有抗氧化、抗發炎等特性；一些文獻表明丹蔘具有穿透房水屏障的能力。然而，關於角膜組織的生物效應尚不清楚。本研究採取紫外線B (UVB) 誘導的角膜損傷的小鼠模型之生物驗證平台，分析探討口服丹酚酸B (SaB) 對炎性損傷的預防效率。將ICR實驗小鼠隨機分為 (1) 空白對照組 (Blank group)；(2) 紫外線B損傷組 (UVB + Vehicle/BID group)；(3) 紫外線B損傷 + 口服丹蔘酚酸B 10 mg/kg/BID組 (UVB + SaB 10 mg/kg/BID group)；(4) 紫外線B損傷 + 口服丹蔘酚酸B 50 mg/kg/BID組 (UVB + SaB 50 mg/kg/BID group)。於實驗末端進行角膜的組織病理學和免疫染色分析。研究結果說明口服丹蔘酚酸B (SaB) 能有效降低基質層發炎細胞的浸潤，維持角膜內皮細胞的型態與密度，另指出口服給藥劑量50mg/kg/BID組比10mg/kg/BID組具有更好的保護效率。研究結果表明丹蔘酚酸B (SaB) 具有防止UVB輻射介導的角膜變性作用，也提供了其生物體內功效的證據。中文摘要 (二) 黃耆 (Astragalus) 為傳統中醫藥常見的用於免疫調節與補氣藥材。黃耆甲苷 (Asg) 是主要的生物活性成分，其作用已經表明在保護心血管、抑制神經炎症和改善中樞神經系統疾病方面。但是，目前尚不清楚黃耆在視功能和視網膜結構保護的功效。本研究在小鼠建立由急性強光誘導的視網膜光損傷模型，用以評估Asg 對視力保護的作用。將ICR實驗小鼠隨機分為 (1) 空白對照組 (Blank group)；(2) 強光損傷組 (Bright light + Vehicle/BID group)；(3) 強光損傷 + 口服黃耆甲苷Ⅳ 50 mg/kg/BID組 (Bright light + Asg 50 mg/kg/BID group)。於實驗期間執行視覺敏銳度 (Visual Acuity, VA) 與視覺對比敏感度功能 (Visual Contrast Sensitivity Function, VCSF) 檢測，並在實驗末端進行視網膜組織結構分析。與載體組相比，結果指出口服Asg (50mg/kg/BID) 可以恢復急性強光照射下的視力 (VA) 和視覺對比敏感功能 (VCSF)，並且還有效地改善視網膜組織結構避免光誘導退化。本研究結果證實Asg對視覺功能保護和視網膜結構保存具有保護作用。

Abstract (一)Salvia miltiorrhiza is a well-known traditional Chinese herbal medicine. It has the properties of promoting blood circulation and removing blood stasis, removing toxic substance, nourishing blood and calming. With the development of modern pharmacology, the isolated product "Salvianolic acid B" (SaB) had been found an effect on anti-oxidation and anti-inflammatory. Some literature presented that Salvia miltiorrhiza had the ability to penetrate the aqueous humor barrier. However, it remained unclear about the bio-effects on the corneal tissue. In this study, a mouse model of ultraviolet B (UVB) induced corneal damage was performed to evaluate the preventive efficiency of inflammatory damage by oral administration of salvianolic acid B (SaB). ICR mice were randomly assigned to (1) Blank group; (2) UVB + Vehicle/BID group; (3) UVB + SaB 10 mg/kg/BID group; (4) UVB + SaB 50 mg/kg/BID group. The histopathologic and immunostaining analysis of cornea were performed at the end of the study. The results showed that oral salvianolic acid B (SaB) could effectively reduce the infiltration of inflammatory cells in the corneal stroma, and also maintain the modality and density of corneal endothelial cells. Moreover, it found that the oral administration dose of 50 mg/kg/BID group had better protective efficiency than the oral dose of 10 mg/kg/BID group. In conclusion, the result of this study demonstrated that Salvianolic acid B (SaB) had the effect of preventing corneal degeneration against UVB radiation-mediated damage, also provided evidence of its efficacy in vivo.Abstract (二)Astragalus root (huangqi) is an important immunomodulatory herb in Chinese medicine. Astragaloside IV (Asg) is the major biological activities ingredient, and the effects had been indicated on protecting the cardiovascular, inhibiting neuroinflammation and improving central nervous system disorders. But, it remains unclear about the efficacy of visual function and retinal structure protection. In this study, a retinal photodamage model induced by acute bright light was established in mice to assess the beneficial effects of Asg on vision protection. ICR mice were randomly assigned to (1) Blank group; (2) Bright light + Vehicle/BID group; (3) Bright light + Asg 50 mg/kg/BID group. The Visual Acuity (VA) and Visual Contrast Sensitivity Function (VCSF) were assessment underlying experimental periods and accompanied by retinal tissue analysis at the end of the study. As compared to the vehicle group, the result indicated that oral administration of Asg (50 mg/kg/BID) could restore the visual acuity (VA) and visual contrast sensitivity function (VCSF) from acute bright light exposure, and also effectively improved the retinal tissue structure against degradation. In this study, it was concluded that Asg had protective effects on visual function protection and retinal structure preservation.