開發一介孔洞矽酸鈣奈米微粒於根管填充材料使用

以矽酸鈣為主的材料在臨床應用上扮演了很重要角色，此類的材料不僅可以誘導骨/牙骨質組織再生，甚至還可以抑制細菌的生長能力。本研究的目的是製備出一新式介孔洞矽酸鈣奈米微粒（mesoporous calcium–silicate，MCS），除了具有優異的磷灰石礦化性質，甚至具有良好的成骨性質、藥物載體釋放和抗菌特性等特性，以符合用於牙髓充填材料的應用。MCS奈米微粒主要是藉由溶膠-凝膠法所製備而成。此外，介孔洞結構、比表面積、孔洞體積以及奈米微粒的體積和型態都進行了詳細的分析。而磷灰石礦化能力、體外促進細胞分化、藥物傳送釋放機制以及抗菌功能也會有更進一步的研究。結果顯示，本研究中使用此簡便模板的方法所合成出的MCS奈米微粒的大小約為200 nm，並具有極高的表面積和孔洞體積，平均內孔徑約為3.05 nm。另外，在MCS奈米微粒也可被做作藥物載體，以維持慶大霉素的持續釋放。也可從牙本質生成的相關蛋白分析證實MCS奈米微粒可促進細胞分化為牙本質。根據上述的結果顯示，MCS奈米微粒是一具有極佳生物相容性且可促進牙本質組織再生的生醫，未來應可應用於臨床上作為牙髓根管充填材料的使用。

Calcium silicate-based materials were playing an important role in endodontic materials, inducing bone/ cementum tissue regeneration and inhibiting bacterial viability. The aim of this study is to prepare novel mesoporous calcium–silicate (MCS) nanoparticles with excellent apatite-mineralization, as well as osteogenic, drug-delivery and antibacterial characteristics for endodontic materials. The MCS nanoparticles were prepared by sol-gel methods. In addition, the mesoporous structure, specific surface area, pore volume and morphology of MCS nanoparticles were been analyzed. The apatite-mineralization ability, in vitro odontogenic differentiation, drug delivery and antibacterial properties were further investigated. The results indicate that MCS nanoparticles with a size of 200 nm were synthesized using a facile template method and showed a high specific surface area and pore volume, with internal mesopores (average pore size: 3.05 nm). Furthermore, the MCS nanoparticles can be used as a drug carrier to maintain the sustained release of gentamicin. Differentiation into odontoblasts was confirmed by odontogenic-related protein. Based on this work, the MCS nanoparticles are potentially useful endodontic materials for dental pulp tissue regenerative materials with biocompatibility and odontogenesis.