2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38798There is a set of completed and fine balance mechanism in the organism for survival and overcome the challenge of environment at any moment. Many growth factors and cytokines play of regulator in our physiological metabolism. Embryo development was regulated by gene expression in infertility couples treated with in vitro fertilization. Leukemia inhibitory factor (LIF) is an essential factor for implantation and establishment of pregnancy. It is valuable medical topic of search on the mechanisms of LIF for embryo development. We have reported that mouse embryos development was effected by LIF-deficiency in vitro. We also found that some genes change their experssion after LIF-deficiency by analysis with gene chips. We found that the epidermal growth factor (EGF) increased the balstocyst rate after LIF-SiRNA treated. EGF also improved embryo development for mice, rats and other mammalian in other reports. The signal transduction of LIF and EGF may involve the STAT or MAPK pathways in various cells. Many reports were indicated that EGF, LIF and their receptors were detected in embryos, but little of their signal transduction pathway was reported in embryos. The aim of this study is search on the relationships of signal transduction pathways from LIF and EGF at preimplantation stage mouse embryos. We design our project for three parts in this study. The first year, we will confirm the major signal transduction induced from LIF and EGF on preimplantation mouse embryos by detected phosphorylation proteins of STAT or MAPK pathways. The second year, we will analysis the interaction of their STAT3 signal transduction pathway between EGF and LIF on mouse embryo development by LIF-SiRNA and inhibitor of STAT. The third year, we will analysis the interaction of their MAPK signal transduction pathway between EGF and LIF on mouse embryo development by LIF-SiRNA and inhibitor of MAPK. This project will understand the relationship of signal transduction in LIF and EGF on embryo development. Our results will supply advantage information for improvement of embryo culture and IVF treatment on reproductive medicine.生物體為了因應生存環境地時刻變遷，必需要有一套完整且精緻的平衡 機制，才能應付隨時可能發生的變化與挑戰，而許多生長因子及細胞激素 則是扮演調節我們體內代謝的一大功臣。血癌抑制因子對著床前期胚胎發 育至桑葚胚期扮演一個極重要的角色，我們之前證實血癌抑制因子是在著 床前期的胚胎發育中是一個非常重要的因子，根據我們近年來的研究及其 他學者的文獻也發現表皮生長因子除了對小鼠胚胎發育有幫助，對大鼠和 其他哺乳類動物的發育都有正向調節的效果。表皮生長因子與血癌抑制因 子間可能共用的訊息傳遞路徑為STAT 及MAPK。許多報告已證明皮生長 因子及血癌抑制因子或其接受體可在胚胎中被偵測到，但是確實的訊息傳 遞路徑並未有報導。本計劃將分三部份進行，首先確定正常發育之胚胎中 血癌抑制因子及表皮生長因子訊息傳遞路徑，透過訊息傳遞物質之抑制劑 的進ㄧ歩觀察這些路徑對對胚胎發育的影響。第二部份探討以干擾性RNA 抑制血癌抑制因子後觀察其訊息傳導路徑受影響的情形，藉由STAT3 之抑 制劑，添加表皮生長因子觀察胚胎發育的路徑是否經由STAT3 的路徑來代 償血癌抑制因子的表現路徑。第三部份則藉由MAPK 之抑制劑，探討以干 擾性RNA抑制血癌抑制因子後添加表皮生長因子觀察胚胎發育的路徑是否 經由MAPK 的路徑來代償血癌抑制因子的表現路徑。經由本研究應可確認 血癌抑制因子在胚胎中的訊息傳遞與上皮生長因子間的關聯性，據以提供 血癌抑制因子及表皮生長因子相互間對胚胎發育的機轉，據以提供胚胎體 外培養環境的改善，而此種資訊亦將有助提高臨床醫師在不孕症治療方面 的治癒率。Project