2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38618An ideal root canal sealer should be nonirritating to the surrounding tissues. Good tissue compatibility is decisive for root canal sealers because they may come into direct contact, especially when extruded, with the periapical tissues. The use of root canal sealers in clinical cases with apical lesions requires special attention to avoid possible interference and/or delay of the healing processes. Unfortunately, all histological investigation demonstrated that all types of root canal sealers could induce mild to severe inflammatory alternations within apical tissues. In recent our research's demonstration root canal filling materials will induce the human bone cell (U2OS): c-fos and c-jun protooncogenes expression; as well as Cyclooxygenase -2 (COX-2) m-RNA gene and protein expression. Known in becomes inflamed under the condition, the massive COX-2 will be induced the production, activation prostaglandin from fatty cell's production, the research to demonstrate that the function may by way of increase osteoclast production member in osteoblast's receptor actvator of NF-κ B ligand (RANKL) take promotes the hemopoiesis cell differentiation as osteoclast, creates result which the bone will absorb.But our present research data demonstration root canal filling materials will induce human bone cell (U2OS) RANKL the m-RNA gene and the protein expression.Recently some research pointed out transcription factor NF-κ B (nuclear factor kappa B) regulative and COX-2 and the bone absorption has the close relationship, moreover the experimental result also showed that when becomes inflamed responds produces, in the cell important news conduction way MAPK (mitogen-activate protein kinase) pathway will be activated.But we study also discovered that the root canal filling agent may induce the human bone cell to produce interleukin-6 and interleukin-8 gene and the protein expression.The evidence demonstrated that possibly is the root canal filling agent tempts apical tissues inflammation to respond, but apical tissues has cytokine activation expression in the inflammation process.This is only the research which we formerly did obtains news. The root canal filling materials influence which creates regarding the human bone tissue, its suggestion molecular action turning point still waited for defining clearly until now, this research plans to be divided three years take the human bone cell (U2OS) as the research model, discusses the root canal filling materials induce the human bone cell news transmission way turning point, by RT-PCR, ELISA,Western blot, protein kinase assay, method discussions and the inflammation related MAPK family signal transduction circuit and the transcription factor and so on electrophoretic mobility shift assay (EMSA) NFκB, as well as the related RANKL/OPG Osteoclast and Osteoblast factor, regulate osteoclasts bone resorption activity. The exploration of mechanism involved in induced of RANKL in human osteoblast by various types root canal filling materials, then deduces various types root canal filling materials regarding periapical tissues infection repair process possible influence; As well as causes the periapical tissues pathological change's turning point. The purpose of this study will investigate cells response to application of root canal sealers induced osteoblast's factor or osteoclast's factor the multiple coefficient of correlation.理想根管充填劑應該對根尖周遭組織是無刺激性。但非常遺憾的是所有組織學的研究，都顯示各種 類型的根管充填劑都會對根尖周圍組織造成不同程度的發炎反應。近年來我們的研究的顯示根管充填 劑，會誘發人類骨細胞(U2OS)中： c-fos 和c-jun protooncogenes 表現；以及環氧化酵素(Cyclooxygenase -2;COX-2)的m-RNA 基因和蛋白質的表現。已知在發炎狀態下，大量環氧化酵素會被誘發生成，活化 前列腺素(prostaglandin)自脂肪細胞的生成，研究顯示作用於造骨細胞的PGE2 可經由增加蝕骨細胞生 成分子(receptor actvator of NF-κB ligand, RANKL)以促進造血細胞分化為蝕骨細胞，造成骨吸收的結 果。而我們的目前研究數據顯示根管充填劑，會誘發人類骨細胞(U2OS)中RANKL 的m-RNA 基因和 蛋白質的表現。近來有研究指出轉錄因子NF-κB(nuclear factor kappa B)的調控與COX-2 和骨吸收有 著密切的關係，另外實驗結果也顯示當發炎反應產生時，細胞內重要的訊息傳導路徑 MAPK(mitogen-activate protein kinase) pathway 會被活化。而我們研究也發現根管充填劑可誘發人類骨 細胞產生interleukin-6 and interleukin-8 gene 與蛋白質的表現。證據顯示可能是根管充填劑誘發根尖組 織發炎反應，而根尖組織在發炎過程中產生組織激素(cytokine) 的活化表現。這只是我們先前所作的 研究所得到一點訊息。 根管充填材料對於人類骨組織造成的影響，其誘發分子作用機轉迄今仍有待厘清，本研究擬分三 年以人類骨細胞（U2OS）為研究模型，來探討根管充填材料誘發人類骨細胞的細胞訊息傳遞路徑機 轉，以RT-PCR、ELISA 、Western blot、protein kinase assay、electrophoretic mobility shift assay (EMSA) 等方法，第一年：探討與RANKL/OPG 有關的蝕骨與成骨因子；第二年：探討與炎症有關的MAPK 家 族信號轉導通路；第三年：研究與轉錄因子NFκB有關的機轉。探討各種類根管充填劑對骨細胞生長、 分化、礦物化的效應，進而推論各種類根管充填劑對於根尖病灶修復過程中可能的影響；以及導致根 尖組織病變的機轉，加以厘清各種類根管充填劑與造骨因子或骨頭破壞相關因子的關連性。根管充填劑蝕骨細胞生成分子蝕骨細胞抑制因子造骨細胞蝕骨細胞root canal sealersRANKLOPGOsteoblastOsteoclastProject